C9017

Concanavalin A from Canavalia ensiformis (Jack bean)

Sepharose^™ conjugate, buffered aqueous suspension

• 동의어(들): ConA
• UNSPSC 코드: 12161501
• NACRES: NA.32

속성

• 생물학적 소스: Canavalia ensiformis
• Quality Level: 200
• 결합: Sepharose^™ conjugate
• 형태: buffered aqueous suspension
• 포함: 20% ethanol as preservative
• 라벨링 범위: 10-16 mg per mL
• 기질: Sepharose 4B
• 기질 활성: cyanogen bromide
• 기질 부착: amino
• 기질 스페이서: 1 atom
• 용량: ≥25 mg/mL binding capacity (thyroglobulin)
• 저장 온도: 2-8°C

설명

애플리케이션

Used for the purification of glycoproteins containing mannose.

생화학적/생리학적 작용

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

물리적 형태

Suspension in 0.1 M acetate buffer, pH 6.0, containing 1 M NaCl, and 1 mM each of CaCl₂ , MgCl₂, and MnCl₂

분석 메모

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

법적 정보

Sepharose is a trademark of Cytiva

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H317,H334,H361
• 예방조치 성명서: P202 - P261 - P272 - P280 - P302 + P352 - P308 + P313
• Hazard Classifications: Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point (°F): 102.2 °F
• Flash Point (°C): 39 °C