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Merck
모든 사진(1)

주요 문서

C8739

Sigma-Aldrich

Cyclin-Dependent Kinase 5 human

recombinant, expressed in E. coli BL21, 50-100 units/μg protein

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About This Item

MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

재조합

expressed in E. coli BL21

Quality Level

양식

solution

특이 활성도

50-100 units/μg protein

포장

vial of 300 units

배송 상태

dry ice

저장 온도

−70°C

애플리케이션

Human cyclin-dependent kinase 5 has been used in a study to determine its 3′ untranslated region subunit 1 contains regulatory elements that affect transcript stability.

생화학적/생리학적 작용

CDK5 is highly expressed in the nervous system where it phosphorylates a multitude of substrates. CDK5 plays an important role in many processes such as: apoptosis, phototransduction, dopamine and glutamate signalling, neurite outgrowth, neurone migration, induction of acetylcholine receptors, golgi membrane traffic and insulin exocytosis. It has also been implicated in the pathogenesis of amyotrophic lateral sclerosis . It is activated by p35 and p39, and their proteolytic cleavage products, p25 and p29.

단위 정의

One unit will phosphorylate 1.0 picomole of the peptide substrate histone H19-18 per minute at 30 °C at pH 7.4.

물리적 형태

Supplied as a solution in PBS pH 7.4, 1 mM EDTA, 1mM DTT and 1 mg/ml BSA

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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문서 라이브러리 방문

Kwok-On Lai et al.
The Journal of biological chemistry, 290(23), 14637-14646 (2015-04-24)
The maturation and maintenance of dendritic spines depends on neuronal activity and protein synthesis. One potential mechanism involves mammalian target of rapamycin, which promotes protein synthesis through phosphorylation of eIF4E-binding protein and p70 ribosomal S6 kinase 1 (S6K). Upon extracellular
Pharmacological inhibitors of cyclin-dependent kinases
Knockaert M, et al.
Trends in Pharmacological Sciences, 23(9), 417-425 (200)
Silvia Moncini et al.
BMC molecular biology, 8, 111-111 (2007-12-07)
CDK5R1 plays a central role in neuronal migration and differentiation during central nervous system development. CDK5R1 has been implicated in neurodegenerative disorders and proposed as a candidate gene for mental retardation. The remarkable size of CDK5R1 3'-untranslated region (3'-UTR) suggests
Shu Zhang et al.
PloS one, 10(7), e0131833-e0131833 (2015-07-07)
Cyclin-dependent kinase 5 (CDK5) is a cytoplasmic serine/ threonine kinase. Knockdown of CDK5 enhances paclitaxel sensitivity in human ovarian cancer cells. This study explores the mechanisms by which CDK5 regulates paclitaxel sensitivity in human ovarian cancers. Multiple ovarian cancer cell

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