H8629

Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

lyophilized powder

• 동의어(들): HK/G6PD
• MDL number: MFCD00131322
• UNSPSC 코드: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

속성

• 형태: lyophilized powder
• glucose-6-phosphate dehydrogenase activity: ≥75 units/mg protein (biuret)
• hexokinase activity: ≥65 units/mg protein
• 정제법: crystallization
• 저장 온도: −20°C

설명

일반 설명

Research area: Cell signaling

Hexokinase and Glucose-6-phosphate Dehydrogenase are intracellular enzymes found distributed widely in the living cells.

Enzyme Commission numbers 2.7.1.1 + 1.1.1.49

애플리케이션

Hexokinase and Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae) has been used to evaluate the adenylate kinase (Adk), activities of the mutant Adk’s. It has also been used for the determination of glucose and ATP.

생화학적/생리학적 작용

Hexokinase initiates the glycolysis pathway by catalyzing the phosphorylation of glucose to Glucose-6-phosphate in the presence of ATP. Glucose-6-phosphate dehydrogenase plays a role in the subsequent oxidation of Glucose-6-phosphate in the presence of triphosphopyridine nucleotide (TPN). Hexokinase and Glucose-6-phosphate Dehydrogenase both serve as a tool in biochemical and analytical studies for determining glucose, fructose, mannose, ATP, and creatin-kinase activity.

포장

Package sizes are based on hexokinase units.

성분

Mixture of hexokinase and glucose-6-phosphate dehydrogenase

단위 정의

Hexokinase Unit Definition: One unit will phosphorylate 1.0 μmole of D-glucose per min at pH 7.6 at 25 °C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)