C8571

Cathepsin B from human liver

buffered aqueous solution, ≥1,500 units/mg protein (E1%/280)

• 동의어(들): Cathepsin B₁
• CAS Number: 9047-22-7
• 효소 위원회 번호: 3.4.22.1 (BRENDA, IUBMB)
• MDL number: MFCD00130748
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human liver
• Quality Level: 200
• 양식: buffered aqueous solution
• 특이 활성도: ≥1,500 units/mg protein (E1%/280)
• UniProt 수납 번호: P07858
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... CTSB(1508)

설명

일반 설명

Cathepsin B gene is mapped to human chromosome 8p23.1. It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence. It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.

애플리케이션

Cathepsin B from human liver has been used:

• as a positive control in the screening Cathepsin B inhibitor assay
• in wall extension (expansin activity) assay
• in microinjection human foreskin fibroblasts to test its effect on apoptosis induction

생화학적/생리학적 작용

Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC). It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases.

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells.

포장

Package size based on protein content

단위 정의

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

물리적 형태

Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable