추천 제품
생물학적 소스
human
Quality Level
재조합
expressed in Nicotiana tabacum (tobacco)
분석
>90% (SDS-PAGE)
형태
liquid
농도
2.5-3.5 mg/mL protein (Sircol method)
기술
cell culture | mammalian: suitable
배송 상태
wet ice
저장 온도
2-8°C
유전자 정보
human ... COL1A1(1277) , COL1A2(1278)
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일반 설명
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
물리적 형태
Supplied as a filter-sterilized liquid in 10 mM HCl. Do not freeze.
제조 메모
Coating of tissue culture dishes: Use 3 to 10 microgram per 1 cm2 dish area. For each cell type and plate type an optimization may be required. Dilute in 10 mM HCl or PBS. Apply the diluted collagen to a tissue culture plate and allow to dry overnight in a laminar flow hood with the plate lid open. Aspirate gently the remaining liquid from the coated wells. Wash twice gently with PBS.
To prepare a collagen gel the fibrillogenesis buffer contains 162 mM sodium phosphate dibasic (Na2HPO4) adjusted to pH 11.2 with 10 N NaOH, filter sterilized. Mix 9 volumes of Collagen at a concentration of 0.3%-1% with 1 volume of fibrillogenesis buffer. Mix well and incubate for 4 to 16 hours at 25°C to 27 °C. Higher concentration of collagen solution might need optimization. Thiis preparation will not spontaneously form a gel in PBS or cell culture medium.
Preparation of collagen sponge: concentrate gel to 10 mg/ml - 100 mg/ml by centrifugation, freeze dry. Crosslink by common methods found in the scientific literature such as 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC), Glutaraldehyde or thermal dehydration.
Other forms: membranes, sheets, fibers can be prepared by methods commonly used with other types of collagen.
To prepare a collagen gel the fibrillogenesis buffer contains 162 mM sodium phosphate dibasic (Na2HPO4) adjusted to pH 11.2 with 10 N NaOH, filter sterilized. Mix 9 volumes of Collagen at a concentration of 0.3%-1% with 1 volume of fibrillogenesis buffer. Mix well and incubate for 4 to 16 hours at 25°C to 27 °C. Higher concentration of collagen solution might need optimization. Thiis preparation will not spontaneously form a gel in PBS or cell culture medium.
Preparation of collagen sponge: concentrate gel to 10 mg/ml - 100 mg/ml by centrifugation, freeze dry. Crosslink by common methods found in the scientific literature such as 1-Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC), Glutaraldehyde or thermal dehydration.
Other forms: membranes, sheets, fibers can be prepared by methods commonly used with other types of collagen.
법적 정보
Collage is a trademark of CollPlant Ltd.
Storage Class Code
13 - Non Combustible Solids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Collagen: The Fibrous Proteins of the Matrix
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