C3867

Rat Collagen Type I

from rat tail, liquid, 4 mg/mL, suitable for cell culture

• 동의어(들): Collagen from rat tail, Col1a1
• CAS Number: 9007-34-5
• EC Number: 232-697-4
• MDL number: MFCD00130825
• UNSPSC 코드: 12352202
• NACRES: NA.75

속성

• 제품명: Collagen, Type I solution from rat tail, BioReagent, suitable for cell culture, sterile-filtered
• 생물학적 소스: rat tail
• Quality Level: 200
• 설명: Approx. 100mg/vial ((35 - 40 ml) )
• 무균: sterile-filtered
• 제품 라인: BioReagent
• 분석: >95% (SDS-PAGE)
• 양식: liquid
• 분자량: apparent mol wt 115-130 kDa by SDS-PAGE (doublet); apparent mol wt 215-235 kDa by SDS-PAGE (doublet)
• 포장: pkg of 1 vial
• 농도: >0.5 mg/mL Biuret
• 기술: cell culture | mammalian: suitable
• 표면 범위: 6‑10 μg/cm²
• 불순물: Mycoplasma Test by Barile, none detected
• solubility: soluble, clear to hazy, colorless (solubilized in 0.02M acetic acid)
• NCBI 수납 번호: NM_053304.1
• UniProt 수납 번호: P02454
• 결합 특이성: Peptide Source: Collagen; ; Peptide Source: Fibronectin
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• 유전자 정보: rat ... Col1a1(29393)

설명

일반 설명

Collagen is an extracellular matrix protein comprising triple helical structure. The structure of collagen type I, in particular, is characterized by the presence of three identical α-chains in its homotrimetric form. Among these three α-chains, one exhibits slight sequence variation. Type I collagen was first isolated from rat-tail tendon, has two main α chains (α and α ) and one β chain.

애플리케이션

Collagen, Type I solution from rat tail has been used for coating culture plates and dishes for human hepatocyte HepG2 cell line and podocytes. It has also been used as a standard for total collagen content calibration curve generation for the quantification of liver extracellular matrix pre-gel.

Used as a coating material to support adherent cells growth and differentiation.

생화학적/생리학적 작용

Collagen protein is essential for the mechanical integrity of tendons and bone. Rat tail tendon collagen is used in tissue engineering especially in the generation of 3-D scaffolds based gels. It has low antigenicity and is compatible with human gingival fibroblasts and human oral keratinocytes.

특징 및 장점

• Sourced from rat tail.
• Sterile-filtered to minimize contamination risks.
• Suitable for a wide range of cell culture applications.
• Provides optimal surface coverage (6-10 μg/cm2) for promoting cell adhesion and proliferation.

제조 메모

Recommended for use as a cell culture substratum. May not be suitable for 3-D gel formation.

Sterile solution prepared from rat tail tendons by a modification of the published extraction method ¹. It is supplied as an aqueous solution in 20mM acetic acid with a protein concentration as stated on the bottle. (approx.100mg protein per vial). Protein concentration was estimated by the Biuret method ².
SDS polyacrylamide gel electrophoresis analysis shows the typical band pattern for Type I collagen, with a doublet at apparent molecular weights of 115 and 130kDa and another doublet at 215 and 235kDa. Based on this analysis, the purity of the collagen sample is >95%.

기타 정보

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

When C3867 is first removed from the cooler it will appear gelled. When it is allowed to come fully to 37°C it will appear as a liquid.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable