C6357

Caspase 4 human

recombinant, expressed in E. coli, 5,000 units/mg protein

• 동의어(들): ICE(rel)II, Ich-2, TX
• 효소 위원회 번호: 3.4.22.57 (BRENDA, IUBMB)
• MDL number: MFCD03456356
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 재조합: expressed in E. coli
• 양식: solution
• 특이 활성도: 5,000 units/mg protein
• 분자량: 30 kDa
• solubility: PBS: soluble
• UniProt 수납 번호: P49662
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... CASP4(837)

설명

일반 설명

The caspase 4 (CASP4) gene is mapped to human chromosome 11q21. Caspase 4 is a cysteine-aspartic protease, localized to the outer membrane of the endoplasmic reticulum (ER). The N-terminus of the enzyme contains a caspase recruitment domain (CARD).

생화학적/생리학적 작용

Caspase 4 is an inflammatory caspase associated with innate immune responses. It plays a key role in the endoplasmic reticulum (ER) stress-induced apoptosis. Caspase 4 mediates the fusion of phagosomes containing pathogens with the lysosome. It inhibits pathogen replication, development, and production of pro-inflammatory cytokines. Caspase 4 induces caspase activation and pyropoptotic death by binding to the intracellular lipopolysaccharide (LPS) of the Gram-negative bacterial outer membrane. This is mediated by the highly specific caspase recruitment domain (CARD).

Useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining target substrates, as well as serving as positive controls in caspase activity assays and Western blot analysis.

단위 정의

One unit will hydrolyze 1 nmol of the caspase substrate WEHD-pNA to WEHD and p-nitroaniline per hour at pH 7.2 at 37 °C.

물리적 형태

Solution in 0.052% ammonium chloride, 0.158% Tris−HCl, and 0.76% sodium chloride

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable