추천 제품
양식
liquid
포장
pkg of 250 ml
기술
cell culture | mammalian: suitable
배송 상태
wet ice
일반 설명
DC Generation Medium (Ready-to-use)
애플리케이션
The Dendritic Cell (DC) Generation Medium has been developed for the easy and efficient generation of immature as well as fully mature myeloid Dendritic Cells from peripheral blood monocytes. DC Generation Medium allows for the generation of DC from cryopreserved as well as freshly isolated cells. In the latter case, the Monocyte Attachment Medium (C-28051) is also needed. The medium consists of a bottle of Basal Medium and one vial of SupplementMix. Adding the SupplementMix to the Basal Medium results in the complete Medium. Recommended for:Human Monocytes (hMo), freshly isolated and cryopreservedHuman Mononuclear Cells (hMNC), freshly isolated
품질
All lots of PromoCell DC Media are subjected to comprehensive quality control tests using human peripheral blood mononuclear cells. Each lot of PromoCell DC Media is tested for the ability to support DC maturation verified by morphological evaluation and flow cytometry analysis. Each lot of PromoCell Monocyte Attachment Medium is tested for its ability to support optimal attachment and viability of peripheral blood Monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
저장 및 안정성
Store the Basal Medium at 4 to 8°C in the dark, store the SupplementMix at -20°C immediately after arrival. Keep the Cytokine Pack at -20°C for long-term storage or at 4 to 8°C for up to 2 weeks. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Complete Medium supplemented with cytokines should be used within 2 weeks. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
계대배양 정규 작업
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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문서
Cryopreserved blood cells and cell culture media for CD34+ human hematopoietic progenitor stem cells, monocytes, PBMCs, macrophages, dendritic and T-cells. Explore over 350 PromoCell products on Sigma.com.
프로토콜
Generate mature human dendritic immune cells from PBMCs or CD34+ stem cell derived monocytes in 7 days in defined and serum-free dendritic cell culture media. Explore over 350 PromoCell products on Sigma.com.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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