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Merck
모든 사진(1)

Key Documents

C-28055

Sigma-Aldrich

M1-Macrophage Generation Medium DXF

250 ml

동의어(들):

Primary Cell Culture Media

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.71

형태

liquid

포장

pkg of 250 ml

기술

cell culture | mammalian: suitable

배송 상태

wet ice

일반 설명

M1-Macrophage Generation Medium DXF

애플리케이션

Macrophage Generation Media have been developed for the efficient generation of monocyte-derived macrophages (MDM) from freshly isolated peripheral blood monocytes or directly from PBMC as a starting material. In the latter case, the Monocyte Attachment Medium (C-28051) is also needed.The M1-Macrophage Generation Medium DXF contains GM-CSF and allows for the generation of M1-polarized macrophages. This xeno-free medium is ready-to-use and includes cytokines for the directed differentiation of M1-like polarized MDM.Recommended for:Human Mononuclear Cells (hMNC), freshly isolatedHuman Monocytes (hMo), freshly isolated

성분

Basal Medium M1 (250ml)SupplementMix M1 (for 250ml)CytokineMix M1: GM-CSF (for 250ml)

품질

All lots of PromoCell Macrophage Media DXF are subjected to comprehensive quality control tests using human peripheral blood mononuclear cells. Each lot of PromoCell Macrophage Media DXF is tested for the ability to support Macrophage differentiation verified by morphological evaluation and flow cytometry analysis. Each lot of PromoCell Monocyte Attachment Medium is tested for its ability to support optimal attachment and viability of peripheral blood Monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

저장 및 안정성

Store the Basal Medium at 4 to 8°C in the dark, store the SupplementMix at -20°C immediately after arrival. Keep the Cytokine Mix at -20°C for long-term storage or at 4 to 8°C for up to 2 weeks. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Complete Medium supplemented with cytokines should be used within 2 weeks. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

계대배양 정규 작업

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Storage Class Code

10 - Combustible liquids


시험 성적서(COA)

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문서 라이브러리 방문

문서

Cryopreserved blood cells and cell culture media for CD34+ human hematopoietic progenitor stem cells, monocytes, PBMCs, macrophages, dendritic and T-cells. Explore over 350 PromoCell products on Sigma.com.

프로토콜

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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