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Merck
모든 사진(1)

주요 문서

B3804

Sigma-Aldrich

BCIP®/NBT-Blue

alkaline phosphatase substrate, chromogenic, liquid

동의어(들):

5-Bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium liquid substrate system

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About This Item

UNSPSC 코드:
12352204
NACRES:
NA.83

제품명

BCIP®/NBT-Blue Liquid Substrate System for Membranes, alkaline phosphatase substrate

Quality Level

양식

liquid

저장 온도

2-8°C

일반 설명

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency.

애플리케이션

BCIP®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity such as western blot analysis.

주의사항

Stable at least one year at 2-8 °C. This substrate is light-sensitive.

기타 정보

Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.

법적 정보

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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