추천 제품
Quality Level
분석
>95%
형태
solution
분자량
Mr 817.7
포장
pkg of 3 mL (300 mg)
제조업체/상표
Roche
기술
Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable
저장 온도
−20°C
일반 설명
NBT is a chromogenic and soluble substance. It is a substrate of alkaline phosphatase (AP) and is converted to an indigo/purple dye on its action.
애플리케이션
- Immunohistocytochemistry
- Western blot
- Southern blot
- Northern blot
- Colony and plaque hybridization
- In situ hybridization
물리적 형태
Solution (100 mg/ml) in 70% dimethylformamide (DMF) (v/v). The color of the NBT solution can vary between yellow and brown depending on the lot used.
Note: The color does not impair the quality or the function of the reagent. In some cases a precipitate may occur which is easily brought into solution by briefly warming the substrate at 37 °C.
Note: The color does not impair the quality or the function of the reagent. In some cases a precipitate may occur which is easily brought into solution by briefly warming the substrate at 37 °C.
제조 메모
Working solution: Preparation of staining solution
- Always prepare this solution fresh shortly before use!For all applications except DIG System: Add 50 μl NBT solution and 37,5 μl BCIP to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2.
- For DIG System applications: Add 50 μl NBT solution and 37,5 μl BCIP to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl
- Note: Do not include MgCl2 in the DIG detection buffer as this might lead to spotty background on the membrane after the detection procedure. Alkaline Phosphatase does not require Mg2+.
기타 정보
For life science research only. Not for use in diagnostic procedures.
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 4 Inhalation - Eye Irrit. 2 - Repr. 1B
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 2
Flash Point (°F)
167.0 °F
Flash Point (°C)
75 °C
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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관련 콘텐츠
There are several counterstains possible in combination with BM Purple (or NBT/BCIP in general), including FastGreen FCF and Nuclear Fast Red.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
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