추천 제품
생물학적 소스
mouse
Quality Level
결합
unconjugated
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
FC82291, monoclonal
양식
buffered aqueous solution
분자량
antigen 37 kDa
종 반응성
rat, kangaroo rat, canine, bovine, human, mouse, monkey, hamster
포장
antibody small pack of 25 μL
농도
~0.5 mg/mL
기술
immunocytochemistry: suitable using 2% formaldehyde-acetone or 10% formalin/methanol-1% NP-40
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.2-0.4 μg/mL using a whole extract of 3T3 (mouse fibroblasts) cells
동형
IgG1
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... NPM1(4869)
mouse ... Npm1(18148)
rat ... Npm1(25498)
일반 설명
B23 (also known as NPM, nucleophosmin, numatrin or NO38) is a phosphoprotein, widely expressed in all cell types, and localized in granular regions of the nucleolus associated with pre-ribosomal particles. This protein was found to be involved in several nuclear functions such as assembly and/or intranuclear transport of preribosomal particles, cytoplasmic/nuclear trafficking, regulation of DNA polymerase A activity and centrosome duplication. Phosphorylation of B23 is mediated by a cyclin complex, CDK2/cyclin E.
Phosphorylation causes the dissociation of B23 from the centrosome and enables the duplication of the centrosome during cell mitosis. B23 was also found to be involved in pro-myelocytic leukemia in which the gene for B23 fuses with retinoic acid receptor a (RARa). As a consequence, a fusion protein (60-7 0 kDa) is produced containing parts of the two genes.
Phosphorylation causes the dissociation of B23 from the centrosome and enables the duplication of the centrosome during cell mitosis. B23 was also found to be involved in pro-myelocytic leukemia in which the gene for B23 fuses with retinoic acid receptor a (RARa). As a consequence, a fusion protein (60-7 0 kDa) is produced containing parts of the two genes.
특이성
It detects both the phosphorylated and the unphosphorylated B23 molecule. The epitope recognized by the antibody lies within the 68 amino acids at the C-terminus of B23.
면역원
rat B23 (nucleophosmin, NPM, numatrin).
애플리케이션
The antibody may be used for ELISA, competitive ELISA, immunoblotting (37 kDa), immunoprecipitation, immunocytochemistry (2% formaldehyde-acetone 1,2,5 or 10% formalin/methanol-1% NP-40 and microinjection (blocks the initiation of centrosome duplication) and immunohistochemistry. Reactivity has been observed with human, monkey, bovine, dog, hamster (weak), rat,kangaroo rat, and mouse B23.
물리적 형태
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
제조 메모
Purified from culture supernatant of hybridoma cells grown in a bioreactor.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Darya M Svistunova et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 60(2), 152-158 (2011-11-25)
It has been demonstrated elsewhere that a high concentration of an antigen within the nucleolus may prevent its proper recognition by specific antibodies. In this study, the authors found that a short proteinase treatment allowed for the detection of antigens
The linker histone H1. 2 is a novel component of the nucleolar organizer regions
Junjie C, et al.
The Journal of Biological Chemistry (2018)
Katharina Thanisch et al.
Nucleic acids research, 42(6), e38-e38 (2013-12-29)
Epigenetic regulation of gene expression involves, besides DNA and histone modifications, the relative positioning of DNA sequences within the nucleus. To trace specific DNA sequences in living cells, we used programmable sequence-specific DNA binding of designer transcription activator-like effectors (dTALEs).
Dynamic localization of alpha-tubulin acetyltransferase ATAT1 through the cell cycle in human fibroblastic KD cells
Nekooki-Machida, et al.
Medical Molecular Morphology, 1-10 (2018)
Jason A Pfister et al.
The Journal of biological chemistry, 291(39), 20787-20797 (2016-08-12)
NPM1 (nucleophosmin 1) is a nucleolar phosphoprotein that regulates many cellular processes, including ribosome biogenesis, proliferation, and genomic integrity. Although its role in proliferating cell types and tissues has been extensively investigated, little is known about its function in neurons
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