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Merck
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Key Documents

B0174

Sigma-Aldrich

AccuTaq LA 10× Buffer

10X Buffer for long and accurate PCR

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About This Item

UNSPSC 코드:
41106306

Grade

for molecular biology

Quality Level

형태

liquid

포장

vial of 0.5 mL

농도

10 ×

기술

PCR: suitable

저장 온도

−20°C

일반 설명

AccuTaq LA 10X buffer is optimized for enhanced amplification of complex templates, when using AccuTaq long and accurate (LA) DNA polymerase.

애플리케이션

AccuTaq LA 10× Buffer has been used:

  • in PCR reaction mixture for the amplification of human BRCA1 gene.
  • as a component of the PCR mix for the amplification of the genomic DNA from Triticum aestivum
  • as a component of the PCR mix for the reverse transcription of RNA from foot and mouth disease virus (FMDV)

법적 정보

AccuTaq is a trademark of Sigma-Aldrich Co. LLC

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Aquatic Chronic 3

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Baozhong Meng et al.
The Journal of general virology, 86(Pt 5), 1555-1560 (2005-04-16)
Grapevine rupestris stem pitting-associated virus (GRSPaV), a member of the genus Foveavirus within the family Flexiviridae, is the putative causal agent of the disease Rupestris stem pitting (RSP) of grapevines. GRSPaV comprises a family of variants whose pathological characteristics have
Jeong-Yun Choi et al.
The Journal of biological chemistry, 286(36), 31180-31193 (2011-07-26)
The hyperthermophilic crenarchaeon Sulfolobus solfataricus P2 encodes three B-family DNA polymerase genes, B1 (Dpo1), B2 (Dpo2), and B3 (Dpo3), and one Y-family DNA polymerase gene, Dpo4, which are related to eukaryotic counterparts. Both mRNAs and proteins of all four DNA
Vandana Jaiswal et al.
PloS one, 10(6), e0129400-e0129400 (2015-06-16)
TaGW2 is an orthologue of rice gene OsGW2, which encodes E3 RING ubiquitin ligase and controls the grain size in rice. In wheat, three copies of TaGW2 have been identified and mapped on wheat homoeologous group 6 viz. TaGW2-6A, TaGW2-6B
Eva-Maria Ladenburger et al.
Molecular and cellular biology, 29(13), 3605-3622 (2009-04-22)
A database search of the Paramecium genome reveals 34 genes related to Ca(2+)-release channels of the inositol-1,4,5-trisphosphate (IP(3)) or ryanodine receptor type (IP(3)R, RyR). Phylogenetic analyses show that these Ca(2+) release channels (CRCs) can be subdivided into six groups (Paramecium
David T Hughes et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(21), 9831-9836 (2010-05-12)
The mammalian gastrointestinal (GI) tract is colonized by a complex consortium of bacterial species. Bacteria engage in chemical signaling to coordinate population-wide behavior. However, it is unclear if chemical sensing plays a role in establishing mammalian host-bacterial commensal relationships. Enterohemorrhagic

프로토콜

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. Red dye allows direct loading of reaction on a gel. REDAccuTaq LA

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