A6229

Anti-AKR1C3 antibody, Mouse monoclonal

clone NP6.G6.A6, purified from hybridoma cell culture

• 동의어(들): Anti-DD-3, Anti-DD3, Anti-HA1753
• MDL number: MFCD06798693
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: NP6.G6.A6, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~38 kDa
• 종 반응성: human
• 포장: antibody small pack of 25 μL
• 기술: immunohistochemistry: suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.25-0.5 μg/mL using cytosolic fraction extract of A549 human lung carcinoma cell
• 동형: IgG1
• UniProt 수납 번호: P42330
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... AKR1C3(8644)

설명

일반 설명

Anti-AKR1C3 antibody, Mouse monoclonal (humanα - hydroxysteroid dehydrogenase, type 2,3; 17β- hydroxysteroid dehydrogenase, type 5) (mouse IgG1 isotype) is derived from the hybridoma NP6.G6. A6 produced by the fusion of mouse myeloma cells (SP20 cells) and splenocytes from BALB/c mice immunized with human AKR1C3 protein. Oxidoreduction of natural and foreign substrates is performed by three enzymes superfamilies, one of them being the AKRs (aldo-keto reductases) family. This family contains 114 proteins that are expressed in prokaryotes and eukaryotes and is divided into 14 subfamilies (AKR1-AKR14). The AKR1 family is the largest among the different fourteen families and contains the aldose reductases, aldehyde reductases, hydroxysteroid dehydrogenases, and steroid 5 β-reductases. AKR1Cs are expressed in many tissues.

면역원

human AKR1C3 protein.

애플리케이션

Anti-AKR1C3 antibody, Mouse monoclonal has been used in

• western blotting
• immunohistochemistry
• enzyme linked immunoassay (ELISA).

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Immunohistochemistry on human placentas were performed using monoclonal mouse anti-AKR1C3 at a dilution of 1:100 or western blot at 1:500.

Mouse monoclonal clone NP6.G6.A6 anti-AKR1C3 antibody used to tag aldo-keto reductase 1C3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of aldo-keto reductase 1C3 in the regulation of the androgen 5α-dihydrotestosterone (DHT) and 3α-diol compound levels in vivo.

생화학적/생리학적 작용

In humans AKR1C3 (aldo-keto reductase family 1 member C3) including AKR1C1,2 and 4, catalyze the reduction of the androgen 5α-dihydrotestosterone (DHT) into inactive 3β or 3α-androstane diol (3α/β-Diol). AKR1C1-4 isoforms also display 3α[17β]-hydroxysteroid oxidase activity using 3α-Diol as a substrate, in vitro. In non-small cell lung carcinoma, AKR1Cs expression is dramatically increased. Due to their product profile and discrete tissue localization, they may regulate the level of active androgens, estrogens, and progestins in target tissues.

표적 설명

AKR1C3, aldo-keto reductase family 1, member C3 (3-α hydroxysteroid dehydrogenase, type II), encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)