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Merck
모든 사진(4)

Key Documents

A6106

Sigma-Aldrich

Aprotinin

BioUltra, 3-8 TIU/mg solid, powder, ≥98% (SDS-PAGE), suitable for inhibition assay

동의어(들):

BPTI, Bovine pancreatic trypsin inhibitor, Trasylol, Trypsin inhibitor (basic)

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About This Item

실험식(Hill 표기법):
C284H432N84O79S7
CAS Number:
Molecular Weight:
6511.44
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.77

product name

Aprotinin from bovine lung, BioUltra, 3-8 TIU/mg solid, ≥98% (SDS-PAGE)

생물학적 소스

bovine lung

제품 라인

BioUltra

분석

≥98% (SDS-PAGE)

형태

powder

특이 활성도

3-8 TIU/mg solid

분자량

~6,500

기술

inhibition assay: suitable

solubility

H2O: 5 mg/mL, clear to very slightly hazy

UniProt 수납 번호

저장 온도

2-8°C

InChI key

ZPNFWUPYTFPOJU-UHFFFAOYSA-N

유전자 정보

cow ... PTI(404172)

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애플리케이션

Aprotinin is largely used as an inhibitor of trypsin.

생화학적/생리학적 작용

Aprotinin is a competitive serine protease inhibitor that forms stable complexes with and blocks the active sites of enzyme. This binding is reversible, and most aprotinin-protease complexes will dissociate at extreme pH levels >10 or <3. Structurally, Aprotinin is a monomeric globular protein derived from bovine lung that consists of 58 amino acids, arranged in a single polypeptide chain with three crosslinking disulfide bridges.

단위 정의

One Trypsin Inhibitor Unit (TIU) will decrease the activity of two trypsin units by 50%, where one trypsin unit will hydrolyze 1.0 μmole of N-alpha-benzoyl-DL-arginine p-nitroanilide per minute at pH 7.8 and 25°C. Another commonly used unit is the KIU, with 1 TIU = 1,300 KIU.
One trypsin inhibitor unit (TIU) will decrease the activity of 2 trypsin units by 50% where one trypsin unit will hydrolyze 1.0 μmole of N-α-benzoyl-DL-arginine p-nitroanilide (BAPNA) per min at pH 7.8 at 25 °C.

제조 메모

A further purification of A1153 to yield a product with slightly higher activity and purity

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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프로토콜

Enzymatic Assay of Aprotinin

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