추천 제품
생물학적 소스
mouse
Quality Level
결합
peroxidase conjugate
항체 형태
purified from hybridoma cell culture
항체 생산 유형
primary antibodies
클론
P5D4, monoclonal
형태
lyophilized powder
농도
1.0-1.5 mg/mL
기술
western blot: 1:1,000 using 20-50 ng of a purified VSV-G tagged fusion protein
동형
IgG1
배송 상태
wet ice
저장 온도
2-8°C
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일반 설명
Monoclonal Anti-VSV-G-Peroxidase is a lyophilized preparation of the purified immunoglobulin fraction of monoclonal Anti-VSV-G (mouse IgG1 isotype) isolated from ascites fluid of the P5D4 hybridoma, conjugated to horseradish peroxidase (HRP). The antibody is derived from the P5D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a synthetic peptide. Vesicular Stomatitis Virus Glycoprotein (VSV-G) exists as a trimer and comprises of four domains, among which domain III is a pleckstrin homology (PH) domain.
특이성
The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein.
VSV-G tag sequence (YTDIEMNRLGK) on VSV-G tagged fusion proteins when expressed N- or C-terminal to the fusion protein. Recognizes native and denatured forms of VSV-G tagged proteins.
면역원
synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.
애플리케이션
Monoclonal Anti-VSV-G−Peroxidase antibody produced in mouse may be used immunoblotting.
생화학적/생리학적 작용
Vesicular Stomatitis Virus Glycoprotein (VSV-G) amino acid sequence YTDIEMNRLGK, corresponding to amino acids 501-511 has been widely used as an epitope tag in expression vectors, enabling the expression of proteins as VSV-G tagged fusion proteins. It constitutes an attractive model to study maturation and intracellular transport of membrane proteins. VSV-G mediates attachment of VSV to the cell surface and induces pH-dependent fusion between viral and target membranes. Besides, its cytoplasmic domain contains information for several intracellular sorting steps, which include efficient export from the endoplasmic reticulum, basolateral delivery and endocytosis..VSV-G is transported through stacks in the Golgi cisternae.
물리적 형태
Supplied as a lyophilized powder. After reconstitution,the solution contains 1% BSA and 0.05% MIT in 0.01 M sodium phosphate buffered saline, pH 7.4.
재구성
Reconstitution with 0.5 mL water results in a solution of 0.01 M sodium phosphate buffered saline, pH 7.4, containing 1% BSA and 0.05% MIT.
저장 및 안정성
Stored the lyophilized product at 2-8 °C. After reconstitution, for extended storage, freeze in working aliquots at –20 °C. For continuous use, the solution may be store at 2-8 °C for up to 1 month. Working dilutions should be discarded. Avoid repeated freeze-thaw.
면책조항
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Skin Sens. 1
Storage Class Code
13 - Non Combustible Solids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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