A1970

Anti-VSV-Glycoprotein−Agarose antibody, Mouse monoclonal

clone P5D4, purified from hybridoma cell culture, PBS suspension

• 동의어(들): Monoclonal Anti-VSV Glycoprotein
• UNSPSC 코드: 12352203
• NACRES: NA.56
• 클론: P5D4, monoclonal
• application: IP; protein purification
• 기술: immunoprecipitation (IP): suitable; protein purification: suitable
• citations: 7

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: agarose conjugate
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: P5D4, monoclonal
• 양식: PBS suspension
• 분석물 화학적 분류: proteins (VSV-G)
• 기술: immunoprecipitation (IP): suitable; protein purification: suitable
• 동형: IgG1
• 용량: ≥15 nmol/mL, resin binding capacity (VSV-G tagged fusion protein)
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Anti-VSV-G Agarose Conjugate is the immunoglobulin fraction of Monoclonal Anti-VSV glycoprotein covalently linked to agarose.

특이성

The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein.

면역원

synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.

애플리케이션

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)

For immunoprecipitation and affinity purification of VSV-G tagged fusion proteins. The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein.

물리적 형태

Supplied as a suspension (1:1,v/v) of beaded agarose in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

제조 메모

Prepared using cyanogen bromide-activated agarose.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable