A5168

Adenosine Deaminase from bovine spleen

Type IX, ammonium sulfate suspension, 150-200 units/mg protein

• 동의어(들): Adenosine aminohydrolase
• CAS Number: 9026-93-1
• 효소 위원회 번호: 3.5.4.4 (BRENDA, IUBMB)
• EC Number: 232-817-5
• MDL number: MFCD00081287
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bovine spleen
• Quality Level: 200
• 유형: Type IX
• 양식: ammonium sulfate suspension
• 특이 활성도: 150-200 units/mg protein
• 분자량: 32.5-33 kDa
• UniProt 수납 번호: P56658
• 외래 활성: 5′-AMP deaminase ≤0.01%; alkaline phosphatase ≤0.01%; guanase ≤0.01%; nucleoside phosphorylase ≤0.1%
• 저장 온도: 2-8°C
• 유전자 정보: cow ... ADA(280712)

설명

일반 설명

Adenosine deaminase/ADA is an enzyme, that is mainly present in lymphocytes and monocytes.

Molecular weight: 32.5-33 kDa

pI: 4.85

애플리케이션

Adenosine Deaminase from bovine spleen has been used to metabolize adenosine triphosphate (ATP) and adenosine to an inactive form (inosine). It has also been used to as an additive in KRH (Krebs−Ringer solution buffered with Hepes, containing CaCl2) medium in order to remove exogenous adenosine for cyclic adenosine monophosphate (cAMP) assay.

Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

생화학적/생리학적 작용

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Adenosine deaminase/ADA can be used as an important marker to identify tuberculous pleural effusion. The activity of ADA is usually high in liver disease, tuberculosis, typhoid, infective mononucleosis and several malignancies.

단위 정의

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

물리적 형태

Suspension in 3.2 M (NH₄)₂SO₄, 0.01 M potassium phosphate, pH 6.0

분석 메모

Protein determined by biuret.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)