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Merck
모든 사진(2)

Key Documents

A4450

Sigma-Aldrich

Monoclonal Anti-β1 and β2-Adaptins antibody produced in mouse

clone 100/1, ascites fluid

동의어(들):

Anti-AP-1 and AP-2

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About This Item

MDL number:
UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

결합

unconjugated

항체 형태

ascites fluid

항체 생산 유형

primary antibodies

클론

100/1, monoclonal

포함

15 mM sodium azide

종 반응성

human, bovine, rat

기술

indirect immunofluorescence: suitable
microarray: suitable
western blot: 1:200 using rat brain extract

동형

IgG1

UniProt 수납 번호

배송 상태

dry ice

저장 온도

−20°C

타겟 번역 후 변형

unmodified

유전자 정보

일반 설명

Monoclonal Anti- β1 and β2-Adaptins (AP-1 & AP-2)(mouse IgG1 isotype) is derived from the 100/1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Adaptins AP-1 and AP-2 are mapped to human chromosome 22q12.2 and 17q12, respectively. AP-1 adaptor consists of four subunits. It includes two large adaptins (one each of γ/α/δ/ε and β1−4 , one medium adaptin (μ1−4), and one small adaptin (ς1−4. Similarly, the AP-2 is made up of two 100-110 kDa polypeptides, termed α and β2 (formerly β) subunits, F2 , and s2 subunit. α and β2 subunits (α and β 2).

면역원

AP-2 adaptor protein from bovine brain.

애플리케이션

Immunofluorescence of MDCK cells fixed in methanol was performed using anti-AP1 at a dilution of 1:250 and also western blot at a dilution of 1:1000.
Monoclonal Anti-β1 and β2-Adaptins antibody produced in mouse has been used in immunoblotting and immunohistology.
Mouse monoclonal anti-β1 and β2-adaptins can be used for western blot (1:200) assays using rat brain extract. The antibody is also suitable for use in indirect immunofluorescence and microarray analyses. Furthermore, monoclonal anti-β1/β2-adaptins have been used for double staining methods to detect clathrin-coated regions of the plasma membrane in rat brain cells.

생화학적/생리학적 작용

β1and β2 adaptins are highly homologous which suggests that the β -type adaptor chains may mediate the interaction of their adaptors with the highly conserved clathrin molecule. Monoclonal antibodies reacting specifically against adaptor proteins are useful tools for studies on the intracellular distribution and structural relationship of adaptor complexes.
Clathrin-coated vesicles facilitate the selective internalization of membrane receptors for lysosomal enzymes from the trans-Golgi network to a pre-lysosomal compartment. Besides clathrin, coated vesicle populations contain the adaptor complexes AP-1 and AP-2, also known as HA-I (HA1) adaptor and HA-II (HA2) adaptor or Assemble Protein 1 (AP1) and Assemble Protein 2 (AP2), respectively. These adaptor proteins mediate the interaction between clathrin and membrane components.

물리적 형태

The antibody is provided as ascites fluid with 0.1% sodium azide as a preservative.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Adaptins: the final recount
Boehm M and Bonifacino JS
Molecular Biology of the Cell, 12(10), 2907-2920 (2001)
Otitis media: a genome-wide linkage scan with evidence of susceptibility loci within the 17q12 and 10q22. 3 regions
Casselbrant ML, et al.
BMC Medical Genetics, 10(1), 85-85 (2009)
Asja Nürnberger et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 52(12), 1647-1655 (2004-11-24)
The dopamine D3 receptor (D3R), intensively studied in neuroscience, also plays an important role in the regulation of renal and cardiovascular function. In contrast to functional findings, less information is available on its localization in the kidney. Neither RT-PCR studies
The structure and function of the beta2-adaptin appendage domain
Owen DJ, et al.
Molecular Biology of the Cell, 19(16), 4216-4227 (2000)
Molecular and genetic profiles of radiographically defined de novo meningiomas
Kitamura Y, et al.
Journal of Neuro-Oncology, 108, 37-44 (2012)

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