추천 제품
생물학적 소스
mouse
결합
unconjugated
항체 형태
ascites fluid
항체 생산 유형
primary antibodies
클론
100/2, monoclonal
포함
15 mM sodium azide
종 반응성
bovine, human, rat
기술
western blot: 1:200 using a crude preparation of synaptic vesicles obtained from rat cerebral cortex
동형
IgG2a
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... AP2A1(160)
rat ... Ap2a1(308578)
일반 설명
Clathrin-coated vesicles facilitate the selective internalization of membrane receptors for lysosomal enzymes from the trans-Golgi network to a pre-lysosomal compartment. Besides clathrin, coated vesicle populations contain the adaptor complexes AP-1 and AP-2, also known as HA-I (HA1) adaptor and HA-II (HA2) adaptor or Assemble Protein 1 (AP1) and Assemble Protein 2 (AP2), respectively. These adaptor proteins mediate the interaction between clathrin and membrane components.
Monoclonal Anti-a-Adaptin (AP-2) (mouse IgG2a isotype) is derived from the 100/2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Adaptor related protein complex 2 subunit alpha 1 (AP2A1) codes for αA adaptin of the AP-2 complex. It is usually seen in clathrin coated vesicles.
The antibody reacts with polypeptides of ~100 kDa in bovine liver, human heart fibroblasts, and Madin-Darby bovine kidney cultured cells (MDBK), but not with any components in the 110-115 kDa range from these sources or from rat pheochromocytoma cultured cells (PC12), neuroblastoma, or astrocytes. This suggests that the 110 and 115 kDa polypeptides may be specific variants that occur only in some cell types of brain. The antibody stains the intact α-adaptor, as well as the 37 and 40 kDa fragments, but not the 63-66 kDa group of α-fragments obtained by trypsin cleavage of the α-subunit molecules.
면역원
AP-2 adaptor polypeptides from bovine brain.
애플리케이션
Immunofluorescence of MDCK cells fixed in methanol was performed using anti-AP1 at a dilution of 1:250 and also western blot at a dilution of 1:1000.
Monoclonal Anti-α-Adaptin antibody produced in mouse has been used in immunoblotting.
Mouse monoclonal anti-α-adaptin antibody has been used:
- as a positive control in immunoblotting assays
- as a primary antibody to detect AP2 levels in tetracycline-repressible stable cell lines
생화학적/생리학적 작용
Adaptor related protein complex 2 subunit α 1 (AP2α1) is very essential for epidermal growth factor receptor (EGFR) nuclear translocation, induced by ultraviolet (UV).
물리적 형태
The product is provided as ascites fluid with 0.1% sodium azide as a preservative.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
The Journal of biological chemistry, 273(27), 17056-17063 (1998-06-27)
Endocytosis of surface proteins through clathrin-coated pits requires an internalization signal in the cytoplasmic domain. Two types of internalization signal have been described: one requiring a tyrosine as the critical residue (tyrosine-based motif), and the other consisting of either two
Molecular cloning of novel transcripts of the adaptor-related protein complex 2 alpha 1 subunit (AP2A1) gene, using Next-Generation Sequencing
Gene, 678, 55-64 (2018)
Virology, 509, 1-10 (2017-06-04)
Acquired Immune Deficiency Syndrome is characterized by a decline in CD4
Abstract 4049: AP2A1 is required for UV-induced EGFR nuclear translocation
Cancer Research, 4049-4049 (2011)
Journal of cell science, 114(Pt 11), 2115-2123 (2001-08-09)
p32/gC1qR is a small acidic protein that has been reported to have a broad range of distinct functions and to associate with a wide array of cellular, viral and bacterial proteins. It has been found in each of the main
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.