A1933

Bovine Serum Albumin

chromatographically purified, New Zealand origin, low endotoxin, suitable for cell culture, pH 7, ≥98%

• 동의어(들): Albumin bovine serum, BSA, Bovine albumin
• CAS Number: 9048-46-8
• EC Number: 232-936-2
• MDL number: MFCD00130384
• UNSPSC 코드: 12352202
• NACRES: NA.27

속성

• 생물학적 소스: bovine
• Quality Level: 200
• 제품 라인: BioReagent
• 분석: ≥98%
• 양식: lyophilized powder
• 분자량: ~66 kDa
• 정제법: chromatography
• 포장: poly bottle of
• 기원: New Zealand origin
• 기술: cell culture | mammalian: suitable
• 불순물: ≤1 EU/mg endotoxin
• pH: 7
• solubility: water: soluble (40 mg/ml)
• UniProt 수납 번호: P02769
• 저장 온도: 2-8°C
• 유전자 정보: bovine ... ALB(280717)

설명

일반 설명

Bovine serum albumin (BSA) is a 66 kDa globular protein crucial for transporting molecules like fatty acids, drugs and hormones from the bloodstream. BSA is a drug carrier for bioactive compounds and has wide clinical applications. BSA has extensive usage in biological research including cell culture, molecular biology, protein biochemistry, and detection techniques. It has wide pharmaceutical applications due to ease of production and interaction flexibility.

애플리케이션

Bovine serum albumin has been used:

• as a standard in bradford protein estimation method in yeast cells
• in elispot assay of blood cells
• as a blocking agent in immunohistochemical studies of transplant
• as a blocking agent in western blotting of human gastric cancer cell lines

생화학적/생리학적 작용

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

특징 및 장점

• Tested for use in cell culture
• Low endotoxin

제조 메모

Prepared by salt fractionation, ion exchange, and gel filtration chromatography.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)