A1233

AMPK (α1/β1/γ1), active, His tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 동의어(들): AMPKG, HAMPKb, MGC17785, MGC33776, MGC57364, MGC8666, PRKAA1, PRKAB1, PRKAG1
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in baculovirus infected Sf9 cells
• 제품 라인: PRECISIO^® Kinase
• 분석: ≥70% (SDS-PAGE)
• 양식: buffered aqueous glycerol solution
• 특이 활성도: 626-848 nmol/min·mg
• 분자량: A1 subunit ~68 kDa; B1 subunit ~38 kDa; G1 subunit ~40 kDa
• solubility: water: soluble
• UniProt 수납 번호: P54619; Q13131; Q9Y478
• 응용 분야: life science and biopharma
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... PRKAA1(5562) , PRKAB1(5564) , PRKAG1(5571)

설명

일반 설명

Research area: CELL SIGNALING

AMP-activated protein kinase (AMPK) is a heterotrimer protein kinase consisting of a α catalytic subunit, and non-catalytic β and γ subunits.

애플리케이션

AMP-activated protein kinase (AMPK) has been used to access the binding kinetics of thalidezine to recombinant active human AMPK using Bio-Layer Interferometry (BLI) binding assay.

생화학적/생리학적 작용

AMP-activated protein kinase (AMPK) is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol.

물리적 형태

Supplied in 50mM NaPhosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1mM PMSF, 0.2 mM DTT, 25% glycerol.

법적 정보

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H360D
• 예방조치 성명서: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter