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Key Documents

75632

Sigma-Aldrich

Osmium tetroxide solution

suitable for electron microscopy, 4% in H2O

동의어(들):

Osmium(IV) oxide

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About This Item

Linear Formula:
OsO4
CAS Number:
Molecular Weight:
254.23
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
NA.47

형태

solution

Quality Level

반응 적합성

reagent type: oxidant

농도

4% in H2O

기술

electron microscopy: suitable

색상

colorless to yellow

pH

6.0-8.0

density

1.0 g/mL at 20 °C

응용 분야

hematology
histology

저장 온도

2-8°C

SMILES string

O=[Os](=O)(=O)=O

InChI

1S/4O.Os

InChI key

VUVGYHUDAICLFK-UHFFFAOYSA-N

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애플리케이션

Osmium tetroxide solution has been used as a fixative in scanning and transmission electron microscopy.

생화학적/생리학적 작용

Osmium tetroxide is also referred to as osmium and is mainly used as a secondary fixative of tissues. The molecule reacts with lipid moieties and is responsible for the oxidation of unsaturated bonds of fatty acids. This reaction adds density and contrast to biological samples. In normal tissues, presence of osmium results in intense black staining which can be viewed by normal eye. In addition, it can be used as a mordant (a molecule which combines with stain or dye at later stages) in electron microscopy, thereby enhancing lead staining.

포장

5 mL in ampule
10 mL in ampule

기타 정보

Due to the hazardous nature of osmium tetroxide, it is recommended to use an ampule opener when using this chemical.

픽토그램

Skull and crossbonesCorrosion

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter


시험 성적서(COA)

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Supercoiled plasmids Col E1 and cDm 506 (a Col E1 derivative carrying the D. melanogaster histone gene repeat) were treated with OsO4 in presence of pyridine and the reaction products were analyzed using different approaches. Gel electrophoresis showed that OsO4
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Journal of Magnetic Resonance Imaging : JMRI, 41, 665-665 (2015)
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