32670

4′,6-Diamidino-2-phenylindole dihydrochloride

suitable for fluorescence, BioReagent, ≥95.0% (HPLC)

• 동의어(들): 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride, DAPI dihydrochloride
• 실험식(Hill 표기법): C₁₆H₁₅N₅ · 2HCl
• CAS Number: 28718-90-3
• Molecular Weight: 350.25
• Beilstein: 4894417
• EC Number: 249-186-7
• MDL number: MFCD00012681
• UNSPSC 코드: 12171500
• PubChem Substance ID: 57648699
• NACRES: NA.32

속성

• 제품 라인: BioReagent
• 분석: ≥95.0% (HPLC)
• 형태: powder
• solubility: DMF: soluble; H₂O: soluble
• 형광: λ_ex 340 nm; λ_em 488 nm (nur DAPI); λ_ex 364 nm; λ_em 454 nm (DAPI-DNA-Komplex); λ_ex 374 nm; λ_em 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA
• 적합성: suitable for fluorescence
• 저장 온도: room temp
• SMILES string: Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
• InChI: 1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
• InChI key: FPNZBYLXNYPRLR-UHFFFAOYSA-N

설명

애플리케이션

DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

생화학적/생리학적 작용

Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.

포장

Bottomless glass bottle. Contents are inside inserted fused cone.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H315,H317,H335
• 예방조치 성명서: P261 - P264 - P271 - P272 - P280 - P302 + P352
• Hazard Classifications: Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)