30915

Denhardt′s solution

BioReagent, for molecular biology, lyophilized powder

• MDL number: MFCD00717549
• UNSPSC 코드: 12352202
• NACRES: NA.52

속성

• Grade: for molecular biology
• 제품 라인: BioReagent
• 형태: lyophilized powder
• 불순물: DNases, none detected; RNases, none detected; phosphatases, none detected; proteases, none detected
• 적합성: suitable for nucleic acid hybridization
• 저장 온도: 2-8°C

설명

일반 설명

Denhardt′s solution is a mixture of high-molecular weight polymers that act as blocking agents. It is used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

애플리케이션

Suitable to block non-specific binding sites in western, northern and Southern blot membranes

성분

Each vial of Denhardt′s powder contains 50 mG Bovine Serum Albumin (BSA), 50 mG Ficoll, and 50 mG Polyvinylpyrrolidone (PVP).

제조 메모

To make a 50x concentrate, dissolve entire contents of vial in 5 mL molecular biology grade water. Store at −20°C for up to two years.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)