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Merck
모든 사진(1)

주요 문서

C7594

Sigma-Aldrich

Casein Blocking Buffer

for Northern and Southern blotting, powder blend

동의어(들):

blocking buffer

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About This Item

EC Number:
UNSPSC 코드:
12161700
NACRES:
NA.25

Grade

for molecular biology

Quality Level

양식

powder blend

기술

Southern blotting: suitable

pH

6.8-7.2(reconstituted per directions)

solubility

H2O: soluble, faintly yellow (dissolves in 5-10 minutes.)

저장 온도

room temp

일반 설명

Casein is a milk-derived substance routinely used in blocking buffers for Western, Southern and Northern Blot procedures. Blocking agents are used to reduce non-specific binding of detection reagents to the membranes.

애플리케이션

Suitable as a blocking Buffer for Western, Southern and Northern Blot membranes. Blocked membranes can then be used in either chemiluminescent, colorimetric, or DIG detection assays.

특징 및 장점

  • Better blocking performance than dried milk or BSA
  • Compatible with neutral and positively-charged membranes
  • Compatible with chemiluminescent, colorimetric , and DIG detection assays

제조 메모

Dissolve entire contents of bottle in 800 mL molecular biology grade water. Stir until well mixed, then add water to final volume of 1L. Store at 2-8°C for up to one week.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1


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Acta medica Okayama, 60(6), 351-355 (2006-12-27)
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Minichromosome maintenance proteins (Mcm) 2, 3, 4, 5, 6 and 7 are related by sequence and form a variety of complexes that unwind DNA, including Mcm4/6/7. A Mcm4/6/7 trimer forms one half of the Mcm2-7 hexameric ring and can be
Ochratoxin A in some French wines: application of a direct competitive ELISA based on an OTA-HRP conjugate.
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Analytical Letters, 42, 1187-1187 (2009)

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