13080801

CHO-K1-AC-free

• UNSPSC 코드: 41106514
• NACRES: NA.81

속성

• 생물학적 소스: hamster ovary
• 설명: Hamster Chinese Ovary, serum-free
• 성장 모드: suspension
• 형태학: Aggregates in suspension
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 설명

This is a derviative of the CHO-K1 cell line, ECACC catalogue no. 85051005 which has been adapted to grow in the animal component free medium, CHO PF Media (C5467)

배양 배지

CHO PF Media (C5467) + 8mM L-Glutamine (G7513). When freezing cells use freeze medium composed of 50:50 fresh:conditioned medium + 10% DMSO.

계대배양 정규 작업

Maintain cultures between approximately 3-9 x 10⁵ cells/ml; 5% CO₂; 37°C. Cells grow as aggregates in suspension. Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10⁵ cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days.

기타 정보

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable