10072801

KF1 cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC 코드: 41106514
• NACRES: NA.81

속성

• 생물학적 소스: fish fin
• 포장: tube of 5 μg 10072801-DNA-5UG; pkg of vial of cells 10072801-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable

설명

세포주 기원

Koi carp fin cell line, koi herpesvirus isolation

세포주 설명

The KF1 cell line was developed from Koi carp (Cyprinus carpio koi) fin.

포장

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

DNA 프로파일

Not specified

배양 배지

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). If a CO₂ incubator is not available, 10 mM HEPES can be included.

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:4, i.e., seeding at 2 x 104 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 25 °C.

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable