04259

N-Ethylmaleimide

BioUltra, ≥99.0% (HPLC)

• 동의어(들): NEM
• 실험식(Hill 표기법): C₆H₇NO₂
• CAS Number: 128-53-0
• Molecular Weight: 125.13
• Beilstein: 112448
• EC Number: 204-892-4
• MDL number: MFCD00005509
• UNSPSC 코드: 12352106
• PubChem Substance ID: 329747899
• NACRES: NA.26

속성

• 제품 라인: BioUltra
• Quality Level: 200
• 분석: ≥99.0% (HPLC)
• 불순물: insoluble matter, passes filter test
• 무기 잔류물: ≤0.05%
• bp: 210 °C (lit.)
• mp: 43-46 °C (lit.); 44-47 °C
• solubility: methanol: 1 M at 20 °C, clear, colorless
• 음이온 미량물: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤100 mg/kg
• 양이온 미량물: Al: ≤5 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• 흡수: cut-off at 382 nm in methanol at 1 M
• 저장 온도: 2-8°C
• SMILES string: CCN1C(=O)C=CC1=O
• InChI: 1S/C6H7NO2/c1-2-7-5(8)3-4-6(7)9/h3-4H,2H2,1H3
• InChI key: HDFGOPSGAURCEO-UHFFFAOYSA-N
• 유전자 정보: human ... SLC6A3(6531) , SLC6A4(6532)

설명

애플리케이션

N-Ethylmaleimide (NEM) has been used:

• to prepare NEM-modified myosin beads
• to block unmodified free thiols in samples
• as a supplement in IP lysis/wash buffer for in vitro SUMOylation assay

Reagent for the covalent modification of cysteine residues in proteins.

생화학적/생리학적 작용

Augments currents from native M-channels in sympathetic neurons and acts as an opener for KCNQ2, KCNQ4 and KCNQ5 channels.

Sulfhydryl alkylating agent that inactivates NADP-dependent isocitrate dehydrogenase and many endonucleases.

안전성 정보

• 픽토그램: GHS06,GHS05
• 신호어: Danger
• 유해 및 위험 성명서: H300,H311,H314,H317
• 예방조치 성명서: P260 - P272 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Oral - Acute Tox. 3 Dermal - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point (°F): 163.2 °F - closed cup
• Flash Point (°C): 72.9 °C - closed cup
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges