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Merck
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Key Documents

95290

Sigma-Aldrich

Methyl Blue

for microscopy (Bot., Hist.), indicator (pH 9.4-14.0)

동의어(들):

Acid blue 93, Aniline blue water soluble, Cotton blue, Poirriers blue, Water blue

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About This Item

실험식(Hill 표기법):
C37H27N3Na2O9S3
CAS Number:
Molecular Weight:
799.80
색상 지수 번호:
42780
EC Number:
MDL number:
UNSPSC 코드:
12171500
PubChem Substance ID:
NACRES:
MA.02

Grade

for microscopy (Bot., Hist.)
indicator (pH 9.4-14.0)

Quality Level

형태

powder or crystals

기술

microbe id | staining: suitable

pH

9.4-14

solubility

water: 0.01 g/10 mL, blue to very deep blue

εmax

≥200 at 310-320 nm
≥400 at 595-605 nm

응용 분야

diagnostic assay manufacturing
hematology
histology

저장 온도

room temp

SMILES string

[Na+].[Na+].OS(=O)(=O)c1ccc(Nc2ccc(cc2)\C(c3ccc(Nc4ccc(cc4)S([O-])(=O)=O)cc3)=C5/C=CC(\C=C5)=N/c6ccc(cc6)S([O-])(=O)=O)cc1

InChI

1S/C37H29N3O9S3.2Na/c41-50(42,43)34-19-13-31(14-20-34)38-28-7-1-25(2-8-28)37(26-3-9-29(10-4-26)39-32-15-21-35(22-16-32)51(44,45)46)27-5-11-30(12-6-27)40-33-17-23-36(24-18-33)52(47,48)49;;/h1-24,38-39H,(H,41,42,43)(H,44,45,46)(H,47,48,49);;/q;2*+1/p-2

InChI key

MCPLVIGCWWTHFH-UHFFFAOYSA-L

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일반 설명

Methyl Blue (C.l. 42780) is a triaminotriphenylmethane dye with each amino group carrying a monosulfonated phenyl substituent. It is also known as Water blue, Acid blue 93, Cotton blue, Poirriers blue, and Aniline blue water soluble.

애플리케이션

Methyl Blue has been used:
  • to stain leaf disks for callose visualization within the cells
  • to incubate cells for 4′,6-diamidino-2-phenylindole (DAPI)/septa staining
  • as a marker for β-(1-3)-d-glucans or acidic phloroglucinol for the detection of callose and lignin
  • in Mann′s stain as it involves a methyl blue-eosin mixture

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point (°F)

>572.0 °F - Pensky-Martens closed cup

Flash Point (°C)

> 300 °C - Pensky-Martens closed cup

개인 보호 장비

dust mask type N95 (US), Eyeshields, Gloves


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C Sahoo et al.
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Photocatalytic degradation of methyl blue (MYB) was studied using Ag(+) doped TiO(2) under UV irradiation in a batch reactor. Catalytic dose, initial concentration of dye and pH of the reaction mixture were found to influence the degradation process most. The
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A novel flow injection method with resonance light scattering detection was developed for the determination of total protein concentrations. This method is based on the enhancement of RLS signals from Methyl Blue (MB) by protein. The enhanced RLS intensities at

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