추천 제품
Grade
Molecular Biology
Quality Level
형태
liquid
사용
sufficient for ≤125 reactions (04743725001)
sufficient for ≤500 reactions (04743733001)
특징
High Fidelity PCR
dNTPs included
hotstart: no
포장
pkg of 125 U (04743725001)
pkg of 500 U (04743733001 [2x250 U])
제조업체/상표
Roche
농도
50 U/mL (working concentration)
파라미터
72 °C optimum reaction temp.
기술
PCR: suitable
불순물
ribonucleases, none detected
≤50 cfu/mL bioburden
색상
colorless
입력
purified DNA
solubility
water: miscible
저장 온도
−20°C
일반 설명
The Expand High FidelityPLUS PCR System is a blend of Taq DNA Polymerase and a thermostable proofreading protein that lacks polymerase activity.
This enzyme blend is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity.
In addition, this enzyme blend also incorporates dUTP and, in combination with Uracil-DNA Glycosylase, can be used to safeguard PCR reactions from cross contamination.
The fidelity of DNA synthesis with the Expand High FidelityPLUS PCR System shows a 2-fold increase compared to the fidelity obtained with the Expand High Fidelity PCR System and a 6-fold increase compared to the fidelity obtained with Taq DNA Polymerase.
These characteristics make the Expand High FidelityPLUS PCR System blend the product of choice if a larger number of targets need to be amplified at the same time with high yield in combination with improved accuracy. In addition, the product can be used for the efficient labeling of DNA fragments with radioactive or non-radioactive modified nucleotides.
This enzyme blend is designed to amplify fragments up to 5 kb from all types of DNA with high yield and fidelity.
In addition, this enzyme blend also incorporates dUTP and, in combination with Uracil-DNA Glycosylase, can be used to safeguard PCR reactions from cross contamination.
The fidelity of DNA synthesis with the Expand High FidelityPLUS PCR System shows a 2-fold increase compared to the fidelity obtained with the Expand High Fidelity PCR System and a 6-fold increase compared to the fidelity obtained with Taq DNA Polymerase.
These characteristics make the Expand High FidelityPLUS PCR System blend the product of choice if a larger number of targets need to be amplified at the same time with high yield in combination with improved accuracy. In addition, the product can be used for the efficient labeling of DNA fragments with radioactive or non-radioactive modified nucleotides.
애플리케이션
- The Expand™ High FidelityPLUS PCR System is designed for the robust amplification of fragments up to 5 kb from all types of DNA with outstanding yield, specificity, sensitivity, and accuracy. This convenient dNTPack is the product of choice for the development of a new amplification assay or the optimization of an existing assay. PCR
- RT-PCR
- Primer extension
- Carryover prevention
Twofold higher yield and threefold greater fidelity are obtained compared to Taq DNA Polymerase. Use Expand High FidelityPLUS PCR System for:
- PCR up to 5kb
- RT-PCR up to 5kb
특징 및 장점
Expand High FidelityPLUS PCR System is a mixture of Taq DNA polymerase and a DNA polymerase with proofreading activity. The Expand High FidelityPLUS PCR System shows a twofold increase in fidelity compared to the Expand High Fidelity PCR System, and a sixfold increase compared to Taq DNA Polymerase.
By incorporating dUTP, Uracil-DNA Glycosylase can be used to prevent PCR product carryover. Use this kit for efficient labeling of DNA with radioactive or non-radioactive nucleotides. It is optimized for PCR of DNA fragments up to 5kb.
Use 2.5U for a standard 50μl PCR.
By incorporating dUTP, Uracil-DNA Glycosylase can be used to prevent PCR product carryover. Use this kit for efficient labeling of DNA with radioactive or non-radioactive nucleotides. It is optimized for PCR of DNA fragments up to 5kb.
Use 2.5U for a standard 50μl PCR.
- Safeguard against cross-contamination.
- Obtain sixfold higher fidelity and higher yields,
- Efficiently label DNA.
- Cost-effective.
포장
1 kit containing 5 components
품질
Each lot is PCR function-tested using human genomic DNA and EPO primers specific for a 1.8kb PCR product, and tPA primers specific for a 4.8kb PCR product.
단위 정의
Volume Activity: 5 U/μl
기타 정보
For life science research only. Not for use in diagnostic procedures.
법적 정보
Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Expand is a trademark of Roche
키트 구성품 전용
제품 번호
설명
- Expand High FidelityPLUS Enzyme Blend 5 U/μl
- Expand High FidelityPLUS Reaction Buffer, with 7.5 mM MgCl2 5x concentrated
- Expand High FidelityPLUS Reaction Buffer, without MgCl2 5x concentrated
- MgCl2 Stock Solution 25 mM
- PCR Nucleotide Mix
유해 및 위험 성명서
예방조치 성명서
Hazard Classifications
Aquatic Chronic 3
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
does not flash
Flash Point (°C)
does not flash
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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