11418467001

Chymotrypsin Sequencing Grade

from bovine pancreas

• 동의어(들): chymotrypsin, protease
• 효소 위원회 번호: 3.4.21.1 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: bovine pancreas
• Quality Level: 100
• 분석: ≥90%
• 양식: lyophilized (salt-free)
• 특이 활성도: ≥70 units/mg protein (at 25 °C, with ATEE as a substrate)
• 분자량: M_r 25 kDa
• 포장: pkg of 4 × 25 μg
• 제조업체/상표: Roche
• 기술: protein sequencing: suitable
• 최적 pH: 7.0-9.0
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Chymotrypsin Sequencing Grade is isolated as a specific protease in pure form from bovine pancreas.

특이성

Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-terminial of Tyr, Phe, and Trp. Leu, Met, Ala, Asp, and Glu are cleaved at a lower rate. Acts also upon amides and esters of susceptible amino acids. The specificity of Chymotrypsin Sequencing Grade is tested with melittin as a substrate.

애플리케이션

Use Chymotrypsin Sequencing Grade for the hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. It is suitable for peptide mapping, fingerprinting, and sequence analysis.

품질

Purity: Free of impurities that may interfere with the specific cleavage or separation of peptides in reversed-phase HPLC.

제조 메모

Working concentration: The recommended amount of enzyme is 1/200 to 1/20 of the quantity of protein by weight.
Storage conditions (working solution): 2 to 8 °C
A solution in 1 mM HCl can be stored for up to one week at 2 to 8 °C.
Inhibitors: Aprotinin, DFP, PMSF, phenothiazine-N-carbonyl chloride, TPCK, ZPCK, α2-macroglobulin, α1-antitrypsin, soybean trypsin inhibitor, and chymostatin. No inhibition by APMSF.

재구성

Dissolve lyophilized chymotrypsin sequencing grade in 1mM HCl. The proteins to be sequenced are dissolved in digestion buffer (100mM Tris-HCl, 10mM CaCl₂, pH 7.8).

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• 픽토그램: GHS07,GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P280 - P284 - P304 + P340 + P312 - P342 + P311
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable