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Merck
모든 사진(1)

주요 문서

10791156001

Roche

Endoproteinase Glu-C (V8 Protease)

from Staphylococcus aureus V8

동의어(들):

V8 protease, protease v8

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About This Item

효소 위원회 번호:
UNSPSC 코드:
23201100

양식

lyophilized (salt-free)

특이 활성도

20 U/mg

분자량

30 kDa

포장

pkg of 2 mg

제조업체/상표

Roche

최적 pH

8.0-8.5

일반 설명

Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at +37°C with casein as the substrate).
At 25 °C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at 37 °C with casein as the substrate).
Endoproteinase Glu-C is a Staphylococcal serine proteinase. Its inhibitors are DFP, α2-macroglobulin and TLCK.

특이성

Heat inactivation: Endoproteinase Glu-C is inactivated by boiling for ten minutes.

애플리케이션

Use Endoproteinase Glu-C (V8 Protease) for protein structure analysis and for sequence analysis.

생화학적/생리학적 작용

Endoproteinase Glu-C specifically hydrolyzes peptide and ester bonds at the carboxylic side of Glu, or both Glu and Asp, depending on the buffer used.

제조 메모

Activator: The enzyme has its maximal activity in presence of SH-reagents
Working concentration: 1 to 5 mM
Working solution: Recommended solvent is 50 mM ammonium acetate pH 4.0 (2 mg/ml).
Storage conditions (working solution): -15 to -25 °C
The enzyme (2 mg/ml in 50 mM ammonium acetate, pH 4.0) is stable for at least one month, frozen in aliquots and thawed only once.

저장 및 안정성

Store at 2 to 8 °C. (Store dry!)

기타 정보

For life science research only. Not for use in diagnostic procedures.

픽토그램

Exclamation markHealth hazard

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

표적 기관

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

does not flash

Flash Point (°C)

does not flash


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