MAB2247

Anti-Integrin β1 Antibody, clone 12G10

clone 12G10, Chemicon^®, from mouse

• 동의어(들): Integrin beta-1, CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 12G10, monoclonal
• 종 반응성: human
• 제조업체/상표: Chemicon^®
• 기술: ELISA: suitable; cell culture | mammalian: suitable; flow cytometry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NM_002211
• UniProt 수납 번호: P05556
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... ITGB1(3688)

설명

일반 설명

Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.

특이성

MAB2247 recognizes the human beta1 integrin. This antibody has been shown to recognize a ligand-induced binding site, with antibody binding increasing in the presence of a fibronectin ligand. This antibody also enhances alpha 5 - beta 1 fibronectin interactions. MAB2247 does not cross-react with mouse or rat samples.

면역원

Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract.

애플리케이션

Anti-Integrin β1 Antibody, clone 12G10 detects level of Integrin β1 & has been published & validated for use in ELISA, FC, IP, FUNC, CULT & WB.

Flow cytometry: 1:25-1:50

ELISA : 10 μg/mL

Western Blot: known to react with human beta 1 integrin subunit under non-reduced conditions only {Mould, 1995}.

Stimulatory to alpha5beta1 interaction with fibronectin {Mould, 1995}.

Optimal working dilutions must be determined by end user.

Research Category
Cell Structure

Research Sub Category
Integrins

표적 설명

88 kDa

물리적 형태

Format: Purified

Protein A purified

Purified immunoglobulin. Liquid in phosphate buffer saline, with 0.1% sodium azide.

저장 및 안정성

Maintain refrigerated at 2–8°C in undiluted aliquots for up one month. For long term storage maintain at -20°C for up to 12 months.

분석 메모

Control
Tonsil, human skin tissue

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable