추천 제품
Quality Level
분석
≥95% (HPLC)
형태
buffered aqueous solution (ready-to-use, 20 mg/ml)
특이 활성도
≥7000 units/mg protein (At 37 °C with pNPP and DEA as the substrates)
포장
pkg of 10 mg (500 μl)
제조업체/상표
Roche
농도
20 mg/mL (ready-to-use)
최적 pH
8.0(Stability)
9.8(Activity)
배송 상태
wet ice
저장 온도
2-8°C
일반 설명
Alkaline phosphatase (ALP) is a hydrolase enzyme. It is a highly active form in calf intestinal mucosa. The calf intestinal alkaline phosphatase is a dimeric glycoprotein with a molecular weight of about 140kDa.
The recombinant, highly active Alkaline Phosphatase possesses the same amino acid sequence as the native, bovine-sourced Alkaline Phosphatase. No animal-derived components are used in the production process. This eliminates the risk of Bovine Spongiform Encephalopathy (BSE) and other infections caused by animals.
The recombinant enzyme guarantees superior lot-to-lot consistency and completely eliminates the risk of BSE.
The recombinant enzyme guarantees superior lot-to-lot consistency and completely eliminates the risk of BSE.
특이성
Alkaline phosphatase catalyzed the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccarides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as enzyme purity, enzyme concentration in the assay, buffer, pH etc.
애플리케이션
Alkaline Phosphatase can be coupled to other proteins via its amino and carbohydrate groups. The enzyme solution can be used directly for conjugation without prior dialysis. For conjugation of AP with IgG, a ration of 2.5:1 is recommended. The application of the conjugated enzyme varies from ELISAs and microarrays to western blot analysis.
생화학적/생리학적 작용
Alkaline phosphatase (ALP) is responsible for removing phosphate groups at the 5- and 3- positions from many types of molecules, such as nucleotides, proteins, and alkaloids. It can also modify lipopolysaccharides by dephosphorylation of the lipid A moiety, thereby making it less toxic than the unmodified lipid A.
규격
≥7,000 U/mg (+37°C; pNPP; DEA)
물리적 형태
Solution in 3 M NaCl, pH 7.6, 5 mM magnesium chloride, 0.1 mM zinc chloride, 30 mM triethanolamine.
제조 메모
Activator: Mg2-, Co2- and Mn2-ions
기타 정보
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
No data available
Flash Point (°C)
No data available
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
이미 열람한 고객
Affinity-chromatography purification of alkaline phosphatase from calf intestine.
The Biochemical Journal, 291-6 null
Intestinal alkaline phosphatase. Physical properties and quaternary structure.
Biochemistry, 1783-8 null
Intestinal alkaline phosphatase detoxifies lipopolysaccharide and prevents inflammation in zebrafish in response to the gut microbiota.
Cell host & microbe, 371-82 null
Quantitation of surface coverage of oligonucleotides bound to chip surfaces: a fluorescence-based approach using alkaline phosphatase digestion.
Lab on a chip, 1332-41 null
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