MABS1812M

Anti-Furin Antibody, clone 16B1.1

clone 16B1.1, from mouse

• 동의어(들): Anti-FUR, Anti-PACE, Anti-PCSK3, Anti-SPC1
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 16B1.1, monoclonal
• 종 반응성: human
• 포장: antibody small pack of 25 μg
• 기술: western blot: suitable
• 동형: IgG2bκ
• NCBI 수납 번호: NP_001276752
• UniProt 수납 번호: P09958
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... FURIN(5045)

설명

일반 설명

Furin (UniProt: P09958; also known as EC: 3.4.2.75, Dibasic-processing enzyme, Paired basic amino acid residue-cleaving enzyme, PACE) is encoded by the FURIN (also known as FUR, PACE, PCSK3) gene (Gene ID: 5045) in human. Furin is an ubiquitously expressed enzyme with endoprotease activity within constitutive secretory pathways and is capable of cleavage at the RX(K/R)R consensus motif. It is single-pass type I membrane protein that shuttles between the trans-Golgi network and the cell surface. It is synthesized with a signal peptide of 26 amino acids and a propeptide region (aa 27-107) that inhibits its activity. The inhibition peptide plays the role of an intramolecular chaperone. It is autocatalytically removed in the endoplasmic reticulum (ER) and remains non-covalently bound to furin as a potent autoinhibitor. Propeptide cleavage is a prerequisite for exit of Furin molecules out of the endoplasmic reticulum. A second cleavage within the propeptide region occurs in the trans-Golgi network, followed by the release of the propeptide and the activation of Furin. Mature Furin has a luminal region (aa 108-705), a helical domain (aa 716-738), and a cytoplasmic region (aa 739-794). The cytoplasmic domain responsible for its trans-Golgi network localization and recycling from the cell surface.

특이성

Clone 16B1.1 detects human Furin and it targets an epitope within 177 amino acids from the C-terminal half.

면역원

GST/His-tagged recombinant fragment corresponding to 177 amino acids from the C-terminal half of human Furin.

애플리케이션

Anti-Furin, clone 16B1.1, Cat. No. MABS1812, is a mouse monoclonal antibody that detects Furin in human cells and has been tested for use in Western Blotting.

품질

Evaluated by Western Blotting in U-251 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Furin in 10 µg of U-251 cell lysate.

표적 설명

~100 kDa observed; 86.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

물리적 형태

Format: Purified

기타 정보

Concentration: Please refer to lot specific datasheet.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1