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Merck
모든 사진(2)

주요 문서

MABN88

Sigma-Aldrich

Anti-K+/Cl- Cotransporter (KCC2) Antibody, clone N1/12

clone N1/12, from mouse

동의어(들):

Electroneutral potassium-chloride cotransporter 2, Erythroid K-Cl cotransporter 2, Neuronal K-Cl cotransporter, electroneural potassium-chloride cotransporter 2, solute carrier family 12, potassium-chloride transporter), member 5

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

N1/12, monoclonal

종 반응성

rat, human

기술

immunohistochemistry: suitable

동형

IgG2aκ

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

일반 설명

KCC2 is a member of the cation-chloride cotransporter gene family. It acts as a K-Cl cotransporter. KCCs normally lower intracellular chloride concentrations below the electrochemical equilibrium potential and depending on the chemical concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. It is proposed to act as the main chloride extruder to promote fast hyperpolarizing postsynaptic inhibition in the brain. KCC2 is expressed at high levels in neurons throughout the nervous system and immunofluorescence shows that the protein is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA′s inhibitory signaling, resulting in motor defects, epilepsy, and anxiety-like behavior.

특이성

Shown by an independent laboratory to react in human and mouse tissue. (UC Davis/NIH NeuroMab Facility Department of Neurobiology, Physiology and Behavior)
This antibody recognizes KCC2

면역원

Epitope: Unknown
Recombinant protein corresponding to rat KCC2.

애플리케이션

Detect K+/Cl-Cotransporter (KCC2) using this Anti-K+/Cl-Cotransporter (KCC2) Antibody, clone N1/12 validated for use in IH.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected KCC2 in rat hypothalamus tissue.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters

품질

Evaluated by Immunohistochemistry in rat cortex area A-3 tissue.

Immunohistochemistry Analysis: 1:300 dilution of this antibody detected KCC2 in rat cortex area A-3 tissue.

표적 설명

126 kDa Calculated

물리적 형태

Format: Purified
Protein G
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Rat cortex area A-3 tissue

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Sana Al Awabdh et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 42(2), 166-182 (2021-11-24)
The K+-Cl- cotransporter KCC2, encoded by the Slc12a5 gene, is a neuron-specific chloride extruder that tunes the strength and polarity of GABAA receptor-mediated transmission. In addition to its canonical ion transport function, KCC2 also regulates spinogenesis and excitatory synaptic function
Vladimir Jovasevic et al.
Nature neuroscience, 18(9), 1265-1271 (2015-08-19)
Fear-inducing memories can be state dependent, meaning that they can best be retrieved if the brain states at encoding and retrieval are similar. Restricted access to such memories can present a risk for psychiatric disorders and hamper their treatment. To
Deepti Chugh et al.
PloS one, 10(7), e0132366-e0132366 (2015-07-16)
Synapsins are pre-synaptic vesicle-associated proteins linked to the pathogenesis of epilepsy through genetic association studies in humans. Deletion of synapsins causes an excitatory/inhibitory imbalance, exemplified by the epileptic phenotype of synapsin knockout mice. These mice develop handling-induced tonic-clonic seizures starting

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