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Merck
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Key Documents

MABF2689

Sigma-Aldrich

Anti-NKG2A/C/E Antibody

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

rat

Quality Level

결합

unconjugated

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

20D5, monoclonal

분자량

observed mol wt ~27.68 kDa

종 반응성

mouse

포장

antibody small pack of 100 μL

기술

flow cytometry: suitable
inhibition assay: suitable

일반 설명

Killer cell lectin-like receptor subfamily C, member 1/2/3 (UniProt: Q9Z202/ Q9WTJ9/Q9QXN7; also known as Natural killer cell protein group 2-A1/ Natural killer cell protein group 2-C1, Natural killer cell receptor NKG2A/Natural killer cell receptor NKG2C/ Natural killer cell group 2E cell receptor NKG2E) are encoded by the Klrc1/2/3 genes (Gene IDs: 16641/ 16642/58179) in murine species. The NKG2 proteins are members of the lectin-like receptor family that heterodimerize with CD94 on the surface of NK and NKT cells. They are also shown to be expressed on activated CD8+ T cells. They are integral component of membrane and are present on the external side of plasma membrane. Within their extracellular carbohydrate recognition domains, NKG2A, NKG2C and NKG2E share extensive homology; however, NKG2C and NKG2E receptors differ significantly from NKG2A in their cytoplasmic domains. NKG2/CD94 heterodimeric complexes are shown to recognize Qa-1 that is described as a nonclassical MHC class I antigen. These heterodimers are reported to transduce signals following ligand binding. NKG2A is shown to be inhibitory in nature, while NKG2C and NKG2E transduce stimulatory signals. Comparatively, NKG2A is more abundant than NKG2C and NKG2E in NK cells. Murine NKG2A contains two intracytoplasmic sequences that closely resemble the Immunoreceptor Tyrosine- based Inhibitory Motif (ITIM). Clone 20D5 can be used for the identification of NK cells expressing functional CD94/NKG2 receptors and is suitable for blocking NKG2A signals in murine tumor models. (Ref.: Vance, RE., et al. (1999). J. Exp. Med. 190(12); 1801-1812).

특이성

Clone 20D5 is a rat monoclonal antibody that detects murine Killer cell lectin-like receptor subfamily C (NKG2 A, C and E). It targets an epitope within the extracellular domain.

면역원

Stable Chinese hamster ovary (CHO) cells expressing the B6 alleles of CD94 and NKG2A.

애플리케이션

Quality Control Testing

Evaluated by Flow Cytometry in C57BL/6 mouse splenocytes.

Flow Cytometry Analysis: 1 μg of this antibody detected NKG2A/C/E in one million C57BL/6 mouse splenocytes.

Tested Applications

Flow Cytometry Analysis: A representative lot detected NKG2A/C/E in Flow Cytometry applications (Vance, R.E., et. al. (1999). J Exp Med. 190(12):1801-12).

Inhibition Analysis: A representative lot of this antibody completely blocked all binding of Qa-1 tetramers to NK1.11 splenocytes. (Vance, R.E., et. al. (1999). J Exp Med. 190(12):1801-12).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-NKG2A/C/E, clone 20D5, Cat. No. MABF2689, is a rat monoclonal antibody that detects Killer cell lectin-like receptor subfamily C, member 1/2/3 and is tested for use in Flow Cytometry and Inhibition Assay.

물리적 형태

Purified rat monoclonal antibody IgG2a in PBS without azide.

저장 및 안정성

Stable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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