MABF125

Anti-TAP1 Antibody, clone mAb 148.3

clone mAb 148.3, from mouse

• 동의어(들): Antigen peptide transporter 1, APT1, ATP-binding cassette sub-family B member 2, Peptide supply factor 1, Peptide transporter PSF1, PSF-1, Peptide transporter TAP1, Peptide transporter involved in antigen processing 1, Really interesting new gene 4 prote
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: mAb 148.3, monoclonal
• 종 반응성: human
• 기술: activity assay: suitable; immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG1κ
• NCBI 수납 번호: NP_000584
• UniProt 수납 번호: Q03518
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... TAP1(6890)

설명

일반 설명

Peptide transporter TAP1 is also called Antigen peptide transporter 1 (APT1), ATP-binding cassette sub-family B member 2 (ABCB2), Peptide supply factor 1 (PSF1), Peptide transporter PSF1 (PSF-1), Peptide transporter involved in antigen processing 1, and Really interesting new gene 4 protein (RING4). TAP1 is involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules as well as MHC class I folding. TAP1 is inhibited herpes simplex virus ICP47 protein, human cytomegalovirus US6 glycoprotein, human adenovirus E3-19K glycoprotein, and is down-regulated by human Epstein-Barr virus vIL-10 protein. TAP1 mutations can be a cause of Bare lymphocyte syndrome 1 (BLS1).

면역원

Epitope: This antibody recognizes the ADAPE amino acid residues within the CYWAMVQAPADAPE sequence of human TAP1 (located at the C-terminus).

Linear peptide corresponding to the CYWAMVQAPADAPE sequence of human TAP1.

애플리케이션

Detect Antigen peptide transporter 1 using this mouse monoclonal antibody, Anti-TAP1 Antibody, clone mAb 148.3 validated for use in western blotting, IP, Immunofluorescence & Activity Assay.

Western Blotting Analysis: A representative lot from an independent laboratory detected TAP1 in transiently transfected HeLa cells (Hulpke, S., et al. (2012). FASEB J. 26(12):5071-5080.).

Western Blotting Analysis: A representative lot from an independent laboratory detected TAP1 in microsomes of baculovirus-infected SF9 cells, which express wild type or select mutations of TAP1 (Chen, M., et al. (2004). J Biol Chem. 279(44):46073-46081.).

Western Blotting Analysis: A representative lot from an independent laboratory detected TAP1 in SF9 cells infected with recombinant baculovirus containing TAP1 gene constructs (Meyer, T. H., et al. (1994). FEBS Lett. 351(3):443-447.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected TAP1 in HeLa cells contransfected with wild type TAP1 and TAP2 (Hulpke, S., et al. (2012). Cell Mol Life Sci. 69(19):3317-3327.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected TAP1 in SF9 cells infected with rBV-TAP1/rBV-TAP2 (Meyer, T. H., et al. (1994). FEBS Lett. 351(3):443-447.).

Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated TAP1 from SF9 cells infected with rBV-TAP1/rBV-TAP2 (Meyer, T. H., et al. (1994). FEBS Lett. 351(3):443-447.).

Activity Assay Analysis: This antibody inhibits TAP-specific peptide transport (Plewnia, G., et al. (2007). J Mol Biol. 369(1):95-107.).

품질

Evaluated by Western Blotting in untreated and Interferon-gamma (IFN-g) treated HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TAP1 in 10 µg of Interferon-gamma (IFN-g) treated HeLa cell lysate.

표적 설명

~65 kDa observed. Uniprot describes a molecular weight of ~87 kDa However, this protein may be observed at ~65 kDa (Koch, J., et al. (2006). FEBS Lett. 580(17):4091-4096.).

물리적 형태

Format: Purified

Purified mouse monoclonal IgG1κ supernatant in buffer containing PBS without preservatives.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable