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Merck
모든 사진(1)

주요 문서

MABE2032

Sigma-Aldrich

Anti-MLK1/MRTF-A Antibody, clone 1A11

동의어(들):

MRTF-A, MKL/myocardin-like protein 1, Megakaryoblastic leukemia 1 protein, Megakaryocytic acute leukemia protein, Myocardin-related transcription factor A

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

1A11, monoclonal

분자량

calculated mol wt 98.92 kDa
observed mol wt ~140 kDa

정제법

using protein G

종 반응성

human

포장

antibody small pack of 100

기술

immunofluorescence: suitable
western blot: suitable

동형

IgG2bκ

에피토프 서열

N-terminal

단백질 ID 수납 번호

UniProt 수납 번호

저장 온도

2-8°C

유전자 정보

human ... MRTFA(57591)

특이성

Clone 1A11 is a mouse monoclonal antibody that detects Myocardin-related transcription factor A (MLK1/MRTF-A). It targets an epitope within the N-terminal region.

면역원

GST-tagged recombinant fragment corresponding to 260 amino acids from the N-terminal region of human Myocardin-related transcription factor A (MLK1/MRTF-A).

애플리케이션

Quality Control Testing

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MLK1/MRTF-A in Ramos cell lysate.

Tested Applications

Immunofluorescence Analysis: A representative lot detected MLK1/MRTF-A in Immunofluorescence application (Busche, S., et al. (2010). J Cell Sci. 123(Pt16):2803-9).

Western Blotting Analysis: A representative lot detected MLK1/MRTF-A in Western Blotting application (Weissbach, J., et al. (2016). Mol Cell Biol. 36(10):1526-39).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

표적 설명

Myocardin-related transcription factor A (UniProt: Q969V6; also known as MRTF-A, MKL/myocardin-like protein 1, Megakaryoblastic leukemia 1 protein, Megakaryocytic acute leukemia protein) is encoded by the MRTFA (also known as KIAA1438, MAL, MLK1) gene (Gene ID: 57591) in human. MRTF-A is a ubiquitously expressed transcription coactivator that associates with serum response factor (SRF) transcription factor to control expression of genes that regulate cytoskeleton during development, morphogenesis, and cell migration. The MRTFA-SRF complex activity responds to Rho GTPase-induced changes in cellular G-actin concentration, and thereby couples cytoskeletal gene expression to cytoskeletal dynamics. It is reported to bind to G-actin via its RPEL repeats. It is detected in cytoplasm as well as in the nucleus and its subcellular location is tightly regulated by actin. High levels of G-actin in the nucleus, observed during serum deprivation, result in low levels of nuclear MRTF-A, while reduced levels of nuclear G-actin result in accumulation of MRTF-A in the nucleus. G-actin-binding in the cytoplasm inhibits its nuclear import by masking the nuclear localization signal (NLS). In contrast, binding to nuclear G-actin promotes its nuclear export to the cytoplasm. Phosphorylation of serine 6 by ERK inhibits the binding of G-actin, unmasking the NLS and promoting nuclear import. Its N-terminal region is required for its nuclear localization and the C-terminal region mediates its transcriptional activity. (Ref.: Weissbach, J., et al. (2016). Mol. Cell. Biol. 36(10); 1526-1539; Busche, S., et al. (2010). J. Cell Sci. 123(16); 2803-2809).

물리적 형태

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

재구성

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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