V9264

Anti-Vinculin antibody, Mouse monoclonal

clone hVIN-1, purified from hybridoma cell culture

• 동의어(들): Monoclonal Anti-Vinculin antibody produced in mouse
• MDL number: MFCD00162905
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: hVIN-1, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen 116 kDa
• 종 반응성: frog, chicken, mouse, canine, human, bovine, rat, turkey
• 포장: antibody small pack of 25 μL
• 농도: ~1.0 mg/mL
• 기술: immunocytochemistry: 5-10 μg/mL using HS-68 human fibroblast cell culture; immunohistochemistry: suitable; indirect ELISA: suitable; western blot: 0.05-0.1 μg/mL using HS-68 total cell extract
• 동형: IgG1
• UniProt 수납 번호: P18206
• 응용 분야: research pathology
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... VCL(7414) ; mouse ... Vcl(22330) ; rat ... Vcl(305679)

설명

일반 설명

Anti-Vinculin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hVIN-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with vinculin purified from human uterus. Vinculin is a cytoskeletal protein associated with the cytoplasmic faces of both cell-cell and cell-extracellular matrix adherens-type junctions. In muscle, vinculin is localized in the fascia adherens of the intercalated disk (cardiac muscle), myotendinous junctions (skeletal muscle), neuromuscular junctions, and the membrane associated dense bodies of smooth muscle.

특이성

Specifically labels vinculin at cell-cell and cell-substrate contacts. Reacts strongly with human vinculin. Shows cross-reactivity with smooth muscle metavinculin.

면역원

purified human vinculin from uterus.

애플리케이션

Anti-Vinculin antibody, Mouse monoclonal has been used in

• immunoblotting
• immunofluorescence staining
• immunocytochemistry
• immunohistochemistry and enzyme linked immunosorbent assay (ELISA).

생화학적/생리학적 작용

Vinculin functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. In many cell types undergoing viral transformation, vinculin becomes redistributed to rosettes or podosomes.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)