A7811
Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse
clone EA-53, ascites fluid
동의어(들):
Actinin Antibody, Sarcomeric Alpha Actinin Antibody, Sarcomeric Alpha Actinin Antibody - Monoclonal Anti-α-Actinin (Sarcomeric) antibody produced in mouse, Anti-1110008F24Rik, Anti-ACTN2, Anti-Actinin α2, Anti-CMD1AA, Anti-MGC107582
About This Item
추천 제품
생물학적 소스
mouse
Quality Level
결합
unconjugated
항체 형태
ascites fluid
항체 생산 유형
primary antibodies
클론
EA-53, monoclonal
분자량
antigen 100 kDa
포함
15 mM sodium azide
종 반응성
mouse, human, frog, pig, feline, chicken, hamster, canine, bovine, fish, snake, rabbit, sheep, goat, rat, lizard
기술
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:800 using human skeletal and cardiac muscle
indirect ELISA: suitable using human and animal muscle tissue
indirect immunofluorescence: suitable using human and animal cultured muscle cells
western blot: 1:2,500 using rat leg muscle
동형
IgG1
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... ACTN2(88)
mouse ... Actn2(11472)
rat ... Actn2(291245)
일반 설명
Mouse monoclonal anti-α-actinin (sarcomeric) antibody stains thymic myoid cells. The antibody exhibits wide reactivity with human and animal muscle tissue.
The antibody is specific for a-skeletal and a-cardiac muscle actinins. The antibody labels Z lines and dots in stress fibers of skeletal muscle in myotubes but does not react with non-skeletal muscle elements (e.g., connective tissue, epithelium, nerves, smooth muscle).
Mouse monoclonal anti-α-actinin (sarcomeric) antibody stains thymic myoid cells. The antibody exhibits wide reactivity with human and animal muscle tissue. α-actinin is detected predominantly in dense bodies and plaques which are characteristic of that tissue. Immunofluorescent labeling of a large variety of cells with anti a -actinin reveals an extensive association of the proteins with the actin containing stress fibers and, in particular, with their membrane-bound termini.
Monoclonal Anti-α-Actinin (Sarcomeric) (mouse IgG1 isotype) is derived from the EA-53 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rabbit skeletal a-actinin.1 The isotype is determined using Mouse Monoclonal Antibody Isotyping Reagents, Catalog Number ISO2. Monoclonal Anti-α-Actinin (Sarcomeric) may be used for the localization of sarcomeric α-actinin using various immunochemical assays such as ELISA, dot blot, immunoblot, immunohistochemistry, and immunocytochemistry. The antibody is useful in the immunolocalization of α-actinin in normal and neoplastic cultured cells and tissues, and for studies on the state of sarcomeric muscle organization, in normal and pathological situations.
특이성
면역원
애플리케이션
생화학적/생리학적 작용
물리적 형태
저장 및 안정성
면책조항
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
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이미 열람한 고객
문서
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