추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
TRA-1-85, monoclonal
종 반응성
human
제조업체/상표
Chemicon®
기술
flow cytometry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
입력
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
동형
IgG1
배송 상태
wet ice
타겟 번역 후 변형
unmodified
일반 설명
The high-frequency blood group antigen Ok(a) is carried on a red cell membrane glycoprotein (gp) of 35-69 kDa that is widely distributed on malignant cells of different origins. Immunostaining of hemopoietic cells and a range of normal human tissues demonstrated a wide distribution of the Ok(a) gp that appears to be nonlineage-restricted, although certain tissues show differentiation-related expression. This clone can be used to monitor contamination of human Embryonic Stem cell cultures with murine feeder cells.
특이성
This antibody exhibits pan-human reactivity, recognizing the cell surface blood group antigen Ok (a). Reactivity to murine cells has not been observed.
SPECIES REACTIVITIES:
Refer to comments regarding specificity
SPECIES REACTIVITIES:
Refer to comments regarding specificity
면역원
2102Ep human Embryonal Carcinoma Cells
Epitope: blood group antigen Ok(a)
애플리케이션
Anti-TRA-1-85 Antibody, blood group antigen Ok(a), clone TRA-1-85 detects level of TRA-1-85 & has been published & validated for use in FC, IP & WB.
FACS Analysis: a starting range of 5-20 μg/ml is suggested.
Immunoprecipitation
Immunoblotting
Optimal working dilutions must be determined by the end user.
Immunoprecipitation
Immunoblotting
Optimal working dilutions must be determined by the end user.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
표적 설명
35-69 kDa
물리적 형태
Format: Purified
Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25 NaCl ph 7.6 with 0.1% Sodium Azide
저장 및 안정성
Maintain refrigerated at 2-8C in undiluted aliquots for up to 12 months
분석 메모
Control
Hemopoietic cells and a wide range of normal human tissues
Hemopoietic cells and a wide range of normal human tissues
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Biochemical and genetic analysis of the OKa blood group antigen.
Williams, B P, et al.
Immunogenetics, 27, 322-329 (1988)
Surface antigens of human embryonic stem cells: changes upon differentiation in culture
Draper J.S. et al.
Journal of Anatomy, 200 (Pt 3), 249-258 (2002)
Jee Myung Yang et al.
Experimental & molecular medicine, 53(4), 631-642 (2021-04-09)
Retinal degenerative disorders, including age-related macular degeneration and retinitis pigmentosa (RP), are characterized by the irreversible loss of photoreceptor cells and retinal pigment epithelial (RPE) cells; however, the long-term effect of implanting both human induced pluripotent stem cell (hiPSC)-derived RPE
Budd A Tucker et al.
eLife, 2, e00824-e00824 (2013-08-31)
Next-generation and Sanger sequencing were combined to identify disease-causing USH2A mutations in an adult patient with autosomal recessive RP. Induced pluripotent stem cells (iPSCs), generated from the patient's keratinocytes, were differentiated into multi-layer eyecup-like structures with features of human retinal
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