MAB4310

Anti-CD133 Antibody, clone 13A4

clone 13A4, Chemicon^®, from rat

• 동의어(들): Prominin-1, AC133 (human)
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rat
• Quality Level: 100
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: 13A4, monoclonal
• 종 반응성: mouse
• 반응하면 안 됨: chicken, Drosophila, rat
• 제조업체/상표: Chemicon^®
• 기술: flow cytometry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 입력: sample type hematopoietic stem cell(s); sample type neural stem cell(s); sample type epithelial cells; sample type: mouse embryonic stem cell(s); sample type mesenchymal stem cell(s)
• 동형: IgG1κ
• 적합성: not suitable for immunohistochemistry (Paraffin)
• NCBI 수납 번호: NM_006017.1
• UniProt 수납 번호: O43490
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: dog ... Prom1(488816) ; human ... PROM1(8842) ; mouse ... Prom1(19126) ; rat ... Prom1(60357)

설명

일반 설명

Prominin is a novel plasma membrane protein with an N-terminal extracellular domain, five transmembrane segments flanking two short cytoplasmic loops and two large glycosylated extracellular domains, and a cytoplasmic C-terminal domain. DNA sequences from Caenorhabditis elegans predict the existence of a protein with the same features, suggesting that prominin is conserved between vertebrates and invertebrates. Prominin is found not only in the neuroepithelium but also in various other epithelia of the mouse embryo. In the adult mouse, prominin has been detected in the brain ependymal layer, and in kidney tubules. In these epithelia, prominin is specific to the apical surface, where it is selectively associated with microvilli and microvilli-related structures. Remarkably, upon expression in CHO cells, prominin is preferentially localized to plasma membrane protrusions such as filopodia, lamellipodia, and microspikes. These observations imply that prominin contains information to be targeted to, and/or retained in, plasma membrane protrusions rather than the planar cell surface. Moreover, results show that the mechanisms underlying targeting of membrane proteins to microvilli of epithelial cells and to plasma membrane protrusions of non-epithelial cells are highly related. {Weigmann, A et al.1997}. Also see {http://www.ncbi.nlm.nih.gov/prow/guide/438375806_g.htm} for more information on CD133 and prominin expression.

특이성

Recognizes mouse Prominin-1 (sometimes also referred to as CD133 and, in the case of the human orthologue, as AC133), a 115-120 kDa pentaspan transmembrane (5-TM) domain glycoprotein. Prominin-1 is expressed on primitive cells such as hematopoietic stem and progenitor cells, neural & endothelial stem cells, retina and retinoblastoma, as well as developing epithelium. To date, the function and ligand of Prominin-1 are unknown.

This antibody can be used to isolate murine stem cells from brain and bone marrow.

The 13A4 antibody does not cross react with rat, human, chicken, or Drosophila antigen. The original paper is located at: http://www.pnas.org/cgi/reprint/94/23/12425.pdf

면역원

Telencephali of ten 12-day-old NMRI mouse embryos (E12, ~1 mg protein), which consist mostly of neuroepithelium, were homogenized in 1 ml PBS, mixed with crushed nitrocellulose filter (1 cm2 in 0.5 ml PBS) as adjuvant, and injected i.p. into a LouXSD rat. {Weigmann et al, PNAS 1997}

애플리케이션

Anti-CD133 Antibody, clone 13A4 detects level of CD133 & has been published & validated for use in FC, IH, IP & WB.

Western Blot Analysis: A 1:1000 dilution from a representative lot detected CD133 in monkey kidney lysate.

Research Category
Stem Cell Research

Research Sub Category
Neural Stem Cells

Hematopoietic Stem Cells

표적 설명

115 kDa

물리적 형태

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Protein A purified

저장 및 안정성

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

분석 메모

Control
Positive Control: Kidney tissue or membranes
Negative Control: Liver tissue or membranes

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable