SAB4200473

Anti-EpCAM ICD antibody, Mouse monoclonal

clone 4A7, purified from hybridoma cell culture

• 동의어(들): Monoclonal Anti-DIAR5, Monoclonal Anti-EGP-2, Monoclonal Anti-EGP314, Monoclonal Anti-EGP40, Monoclonal Anti-ESA, Monoclonal Anti-EpCAM ICD antibody produced in mouse, Monoclonal Anti-HNPCC8, Monoclonal Anti-KS1/4, Monoclonal Anti-KSA, Monoclonal Anti-M4S1, Monoclonal Anti-MIC18, Monoclonal Anti-MK-1, Monoclonal Anti-TACSTD1, Monoclonal Anti-TROP1, Monoclonal Anti-epithelial cell adhesion molecule
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: 4A7, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~40 kDa
• 종 반응성: human, mouse
• 농도: ~1.0 mg/mL
• 기술: indirect immunofluorescence: 5-10 μg/mL using human MCF7 cells.; western blot: 0.25-0.5 μg/mL using whole extracts of human SW480 cells.
• 동형: IgG1
• UniProt 수납 번호: P16422
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... EPCAM(4072) ; mouse ... Epcam(17075)

설명

일반 설명

Monoclonal Anti- EpCAM ICD (mouse IgG1 isotype) is derived from the hybridoma 4A7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the intracellular domain of human EpCAM, conjugated to KLH. Epithelial cell adhesion molecule (EpCAM) is an epithelial transmembrane glycoprotein, composed of an ectodomain, a transmembrane domain and a cytoplasmic domain. EpCAM is frequently expressed in human malignancies, normal stem and progenitor cells, and at lower levels, in normal epithelia.

면역원

synthetic peptide corresponding to the intracellular domain of human EpCAM, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

애플리케이션

Monoclonal Anti-EpCAM ICD antibody has been used in

• immunoblotting
• immunoprecipitation
• immunofluorescence

생화학적/생리학적 작용

Epithelial cell adhesion molecule′s (EpCAM) intracellular domain (ICD) fragment translocates to the nucleus after cleavage from EpCAM ectodomain and associates with other molecules to bind DNA resulting in cell proliferation and tumor formation.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable