추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
ascites fluid
항체 생산 유형
primary antibodies
클론
2HP-1H5, monoclonal
종 반응성
mouse, human
제조업체/상표
Chemicon®
기술
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
동형
IgG1κ
적합성
not suitable for Western blot
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... CBX5(23468)
일반 설명
Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.
특이성
Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.
면역원
Recombinant mouse HP1a.
애플리케이션
Immunocytochemistry: 1:500-1:5,000
Immunohistochemistry: 1:500-1:5,000
ELISA: 1:500-1:5,000
Western blot: not recommended. For Western blot use MAB3446.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:500-1:5,000
ELISA: 1:500-1:5,000
Western blot: not recommended. For Western blot use MAB3446.
Optimal working dilutions must be determined by the end user.
Use Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-1H5 (mouse monoclonal antibody) validated in ELISA, ICC, IHC to detect Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5.
물리적 형태
Liquid ascites no preservative.
분석 메모
Control
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Leucine-rich repeat and WD repeat-containing protein 1 is recruited to pericentric heterochromatin by trimethylated lysine 9 of histone H3 and maintains heterochromatin silencing.
Chan, KM; Zhang, Z
The Journal of Biological Chemistry null
Diverse factors are involved in maintaining X chromosome inactivation.
Chan KM, Zhang H, Malureanu L, van Deursen J, Zhang Z
Proceedings of the National Academy of Sciences of the USA null
Interchromosomal associations between alternatively expressed loci.
Charalampos G Spilianakis, Maria D Lalioti, Terrence Town, Gap Ryol Lee, Richard A Flavell
Nature null
Targeting polycomb to pericentric heterochromatin in embryonic stem cells reveals a role for H2AK119u1 in PRC2 recruitment.
Cooper, S; Dienstbier, M; Hassan, R; Schermelleh, L; Sharif, J; Blackledge, NP; De Marco et al.
Cell Reports null
DNA methylation promotes Aurora-B-driven phosphorylation of histone H3 in chromosomal subdomains.
Monier, K; Mouradian, S; Sullivan, KF
Journal of Cell Science null
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