추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
human, mouse, rat, chicken
제조업체/상표
Upstate®
기술
ChIP: suitable (ChIP-seq)
dot blot: suitable
immunocytochemistry: suitable
inhibition assay: suitable (peptide)
multiplexing: suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
trimethylation (Lys9)
유전자 정보
human ... H3C1(8350)
mouse ... H3C1(360198)
rat ... H3C1(679994)
일반 설명
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
특이성
Expected to cross-react with chicken, mouse and rat.
Trimethyl-histone H3 (Lys9). Broad species cross-reactivity expected.
면역원
Epitope: Lys9
KLH-conjugated, 2X-branched synthetic peptide containing the sequence …AR[me3K]ST… in which me3K corresponds to trimethyl-lysine at residue 9 of human histone H3.
애플리케이션
Anti-trimethyl-Histone H3 (Lys9), Cat. No. 07-442 is a rabbit polyclonal antibody that detects Histone H3 (Met3-Lys9) and is tested for use in Western Blotting, Dot Blot, Peptide Inhibition Assay, and Immunocytochemistry.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Tested Applications
Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Histone H3 (Met3-Lys9) in Mouse Embryo Fibroblasts (MEF).
Peptide Inhibition Assay: Target band detection in acid extract of HeLa cells was prevented by pre-blocking of a representative lot with the immunogen Histone H3 (Met3-Lys9) peptide, but not the corresponding unmodified Histone H3 peptide.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected trimethyl-Histone H3 (Lys9) peptide, but did not detect di-methyl Histone H3 (Lys9) peptide, mono-methyl Histone H3 (Lys9) peptide, nor unmodified Histone H3 (1-19) peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Histone H3 (Met3-Lys9) in Mouse Embryo Fibroblasts (MEF).
Peptide Inhibition Assay: Target band detection in acid extract of HeLa cells was prevented by pre-blocking of a representative lot with the immunogen Histone H3 (Met3-Lys9) peptide, but not the corresponding unmodified Histone H3 peptide.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected trimethyl-Histone H3 (Lys9) peptide, but did not detect di-methyl Histone H3 (Lys9) peptide, mono-methyl Histone H3 (Lys9) peptide, nor unmodified Histone H3 (1-19) peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
품질
Evaluated by Western Blotting in HeLa cell acid extract.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Histone H3-Lys9me3 in HeLa cell acid extract, but did not detect unmodified recombinant Histone H3.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Histone H3-Lys9me3 in HeLa cell acid extract, but did not detect unmodified recombinant Histone H3.
표적 설명
17 kDa
물리적 형태
Format: Purified
Protein A chromatography
Purified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol.
저장 및 안정성
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Control
Acid-extracted proteins from HeLa cells.
Acid-extracted proteins from HeLa cells.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Msc1 links dynamic Swi6/HP1 binding to cell fate determination.
Lawrence, RJ; Volpe, TA
Proceedings of the National Academy of Sciences of the USA null
Short-Chain Fatty Acids Regulate the Enteric Neurons and Control Gastrointestinal Motility in Rats.
Soret R, Chevalier J, De Coppet P, Poupeau G, Derkinderen P, Segain JP, Neunlist M
Gastroenterology null
The transcriptional repressor NIPP1 is an essential player in EZH2-mediated gene silencing.
Nuytten, M; Beke, L; Van Eynde, A; Ceulemans, H; Beullens, M; Van Hummelen, P; Fuks, F; Bollen, M
Oncogene null
John Arne Dahl et al.
Stem cells (Dayton, Ohio), 25(4), 1037-1046 (2007-02-03)
Chromatin immunoprecipitation (ChIP) is a key technique for studying protein-DNA interactions and mapping epigenetic histone modifications on DNA. Current ChIP protocols require extensive sample handling and large cell numbers. We developed a quick and quantitative (Q(2))ChIP assay suitable for histone
Positive histone marks are associated with active transcription from a methylated ICSBP/IRF8 gene.
Marina Tshuikina, Kenneth Nilsson, Fredrik Oberg
Gene null
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