MAB3560

Anti-8-Oxoguanine Antibody, clone 483.15

ascites fluid, clone 483.15, Chemicon^®

• 동의어(들): 8-oxoG
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 100
• 항체 형태: ascites fluid
• 항체 생산 유형: primary antibodies
• 클론: 483.15, monoclonal
• 종 반응성: monkey, human, primate
• 종 반응성(상동성에 의해 예측): mouse, rat
• 제조업체/상표: Chemicon^®
• 기술: ChIP: suitable; ELISA: suitable; immunocytochemistry: suitable
• 동형: IgM
• 배송 상태: dry ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... OGG1(4968)

설명

일반 설명

8-Oxoguanine is a mutagenic oxidative damage product of guanine. Oxidatively damaged base 8-oxoguanine is generated in the DNA of all living organisms due to the presence of reactive oxygen species in cells. DNA damage threatens the health of the genetic information stored in every DNA molecule; however enzymes exist in cells to protect against the mutagenic effect of this lesion. 8-oxoguanine is one of the most abundant and well-characterized DNA lesions generated by oxidative stress. It has been estimated that ~180 guanines are oxidized to 8-oxoG per mammalian cell per day. 8-oxoG is a miscoding lesion that can cause G:C to T:A or T:A to G:C transversion mutations. This lesion accumulates in DNA with age and it has been loosly linked to several cancers and diseases, such as Alzheimer′s and Parkinson′s.

특이성

8-oxoguanine. By competitive ELISA the monoclonal showed no reactivity with dGMP, dAMP, dCMP or TMP in micromolar concentrations. Competition was only observed when the concentrations were increased to the millimolar range.

면역원

8-oxoguanine adsorbed on to alumina

애플리케이션

Anti-8-Oxoguanine Antibody, clone 483.15 detects level of 8-Oxoguanine & has been published & validated for use in ELISA & IC.

Immunocytochemistry:
On HeLa and Cos7 cells fixed with paraformaldehyde. 8-oxoguanine has been localized to the nucleus in nutrient-deprived cells.

ELISA:
Cell grown in slides were extracted twice for 30 sec with cold 560nM NaCl;0.1% (v/v) Triton X-100;0.02% (w/v) SDS;10mM phosphate buffer pH 7.4 and fixed with freshly prepared 4% PFA for 5-20 minutes at room temperature. {Conlon, KA et al (2000) J. Histotechnology 23(1):37-44}. Typical staining shows that nuclei from extracted cells have define periphery and areas of condensed chromatin. Clone 483.15 staining showed specific but faint nuclear fluorescence staining in cells incubated in supplemented DMEM, but in cells incubated in nutrient-free defined solt solution (NFDSS) {1.8mM calcium chloride, 110mM NaCl, 44mM sodium biocarbonate, pH 7.5} showed strong nuclear fluorescence staining that appeared punctate and gernerally distributed. Fluorescence staining disappears to background levels in cells incubated in nutrient medias even after initial NFDSS treatments {Conlon et al}.

A previous lot of this antibody was used in an ELISA assay.

Optimal working dilutions must be determined by end user.

품질

Routinely evaluated by immunocytochemistry on NIH/3T3 cells..

Immunocytochemistry:
Confocal fluorescent analysis of NIH/3T3 cells using anti-8-oxoguanine mouse monoclonal antibody.

물리적 형태

Unpurified mouse monoclonal IgM liquid in buffer containing no preservative.

저장 및 안정성

Stable for 6 months at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgM and affect product performance.

분석 메모

Control
Nutrient starved HeLa or Cos7 cells

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable