추천 제품
생물학적 소스
mouse
Quality Level
항체 형태
culture supernatant
항체 생산 유형
primary antibodies
클론
133A2, monoclonal
종 반응성
rat, mouse, canine, bovine, human
제조업체/상표
Chemicon®
기술
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
동형
IgG3
NCBI 수납 번호
UniProt 수납 번호
배송 상태
dry ice
타겟 번역 후 변형
unmodified
유전자 정보
human ... LMNA(4000)
일반 설명
Nuclear lamins form a network of filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished: A-type lamins and B-type lamins. The A-type lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. lamin A, lamin C and lamin Adel 10, while the B-type lamins include two proteins arising from two distinct genes, i.e. lamin B1 and lamin B2.
특이성
Monoclonal 133A2 reacts against human lamin A but not lamin C. The epitope lies with the 98 amino acids of the C terminus that is unique to lamin A. Specifically deletion analysis has shown that amino acids 598-611 were essential for reactivity {Hozak, P et.al. (1995), J. Cell Sci 635-644}.
면역원
Epitope: C-terminus, a.a. 598-611
Synthetic peptide from the C-terminus of human lamin A. This sequence is not present in the the lamin C isoform.
애플리케이션
Immunoblotting: Lamin A detected as a 70kDa protein under reduced conditions.
Immunocytochemistry on fixed cells (methanol/acetone fixation)
Immunohistochemistry on frozen tissue sections.
Optimal working dilutions must be determined by the end user.
Immunocytochemistry on fixed cells (methanol/acetone fixation)
Immunohistochemistry on frozen tissue sections.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
This Anti-Lamin A Antibody, C-terminus, a.a. 598-611, clone 133A2 is validated for use in FC, WB, IC, IH for the detection of Lamin A.
물리적 형태
Format: Purified
Liquid in buffer containing 0.1% sodium azide.
저장 및 안정성
Maintain -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Raju Padiya et al.
PloS one, 9(5), e94228-e94228 (2014-05-07)
Cardiovascular complication due to diabetes has remained a major cause of death. There is an urgent need to intervene the cardiac complications in diabetes by nutritional or pharmacological agents. Thus the present study was designed to find out the effectiveness
Catherine Coffinier et al.
The Journal of biological chemistry, 285(27), 20818-20826 (2010-05-05)
Lamin A, a key component of the nuclear lamina, is generated from prelamin A by four post-translational processing steps: farnesylation, endoproteolytic release of the last three amino acids of the protein, methylation of the C-terminal farnesylcysteine, and finally, endoproteolytic release
J L Broers et al.
Histochemistry and cell biology, 107(6), 505-517 (1997-06-01)
A selection of normal human tissues was investigated for the presence of lamins B1, B2, and A-type lamins, using a panel of antibodies specific for the individual lamin subtypes. By use of immunoprecipitation and two-dimensional immunoblotting techniques we demonstrated that
P Hozák et al.
Journal of cell science, 108 ( Pt 2), 635-644 (1995-02-01)
The nuclear lamina forms a protein mesh that underlies the nuclear membrane. In most mammalian cells it contains the intermediate filament proteins, lamins A, B and C. As their name indicates, lamins are generally thought to be confined to the
Lamin A is part of the internal nucleoskeleton of human erythroleukemia cells.
Neri, L M, et al.
Journal of Cellular Physiology, 178, 284-295 (1999)
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