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Merck
모든 사진(2)

주요 문서

MAB3420-AF555

Sigma-Aldrich

Anti-Tau Antibody, clone PC1C6, Alexa Fluor 555 Conjugate

clone PC1C6, from mouse, ALEXA FLUOR 555

동의어(들):

Microtubule-associated protein tau, Tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.45

생물학적 소스

mouse

Quality Level

결합

ALEXA FLUOR 555

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

PC1C6, monoclonal

종 반응성

human, rat, bovine, mouse

기술

immunocytochemistry: suitable
immunofluorescence: suitable

동형

IgG2a

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

일반 설명

Microtubule-associated protein tau (UniProt P29172; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as TAU) gene (Gene ID 281296) in bovine species. Extracellular plaque deposits composed of amyloid-β and intracellular neurofibrillary tangles (NFTs) composed of truncated and hyperphosphorylated tau are two neuropathological hallmarks of Alzheimer′s disease (AD). Tau pathology (tauopathy) can cause toxicity when the brain is devoid of amyloid plaques, and tangle pathology correlates better with clinical dementia than amyloid pathology. Abnormal processing of tau by hyperphosphorylation and proteolytic truncation contribute to the toxicity of tau. Tau is known to be cleaved by various proteases, including caspases, calpains, cathepsins, thrombin, and puromycin-sensitive aminopeptidase, as well as the lysosomal cysteine proteinase asparagine endopeptidase (AEP). MAPK, GSK-3, and Cdk-5 are three known kinases that target various tau phosphorylation sites. Tau also undergoes other types of posttranslational modifications, including glycosylation, ubiquitination, glycation, polyamination, nitration, and lysine methylation, which are believed to be important for its non-pathological functions, including polymerization and stabilization of microtubules.

특이성

Clone PC1C6 was originally designated as clone Tau-1. Clone PC1C6 (Tau-1) was initially thought to specifically target a form of tau present in axons by immunohistochemical staining of rat brain tissue (Binder, L.I. et al. (1985). J. Cell Biol. 101(4):1371-1378), later studies revealed that its immunoreactivity and therefore tissue staining pattern depends on the state of tau phosphorylation. Specifically, clone Tau-1 displayed diminished immunoreactivity toward Tau when phosphorylated in its epitope region. Sample dephosphorylation by alkaline phosphatase treatment enhanced the immunoreactivity of this clone in Western blotting and immunohistochemistry applications (Papasozomenos, S.C., and Binder, L.I. (1987) Cell Motil. Cytoskeleton. 8(3):210-216; Szendrei, G.I., et al. (1993). J. Neurosci. Res. 34(2):243-249); Billingsley, M.L., and Kincaid, R.L. (1997). Biochem. J. 323(Pt 3):577-591; Dotti, C.G., et al. (1987). Neuroscience. 23(1):121-130).

면역원

Epitope: Internal (C-terminal half).
Purified denatured bovine microtubule associated proteins.

애플리케이션

Immunofluorescence Analysis: A 1:300 dilution from a representative lot detected Tau in rat brain tissue.
The unconjugated antibody (Cat. No. MAB3420) is shown to be suitable also for Western blotting and immunohistochemistry applications.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
This Anti-Tau Antibody, clone PC1C6, Alexa Fluor 555 Conjugate is validated for use in Immunofluorescence, Immunocytochemistry for the detection of Tau.

품질

Evaluated by Immunofluorescence in mouse brain tissue.

Immunofluorescence Analysis: A 1:150 dilution of this antibody detected Tau in mouse brain tissue.

표적 설명

~79 kDa observed.

물리적 형태

Protein A purified
Purified mouse monoclonal IgG2a antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

기타 정보

Concentration: Please refer to lot specific datasheet.

법적 정보

ALEXA FLUOR is a trademark of Life Technologies

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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